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product-image-AAA50486_AD10.jpg Application Data (Published customer image:Mouse anti human cytokeratin (Pan reactive) antibody, clone C-11 used to label cytokeratins in bovine recto-anal junction tissue sections by immunofluorescence.Image caption:Immunofluorescent images of tissue sections from RAJ-IVOC adherence assay trial 1. The RAJ-IVOC were inoculated with either O157 (106 CFU inoculum as shown in parenthesis) or not inoculated (no bacteria) and incubated at 39 °C for 3 or 24 h. Tissue sections of the RAJ-IVOC were then stained with immunofluorescent antibodies targeting the RAJ cells’ cytokeratins and O157, and images were recorded at 100× magnification. The adherent bacteria (shown with arrows), RAJ cells’ cytokeratins, and the nuclei have green, orange–red, and blue fluorescence, respectively. The squamous (S), junction (J, boxed), and columnar (C) regions of the RAJ are indicated along with a 100 um scale bar.)

Mouse CYTOKERATIN Monoclonal Antibody | anti-KRT6A antibody

MOUSE ANTI HUMAN CYTOKERATIN (PAN REACTIVE)

Reactivity
Human, Mammals
Applications
Immunoprecipitation, Immunohistochemistry, Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay, Immunofluorescence
Purity
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Synonyms
CYTOKERATIN, Antibody; MOUSE ANTI HUMAN CYTOKERATIN (PAN REACTIVE); anti-KRT6A antibody
Ordering
Host
Mouse
Reactivity
Human, Mammals
Clonality
Monoclonal
Isotype
IgG1
Clone Number
C-11
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Form/Format
Purified IgG - liquid
Phosphate buffered saline
<0.1% Sodium Azide (NaN3)
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Applicable Applications for anti-KRT6A antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot), FCM/FACS (Flow Cytometry), IF (Immunofluorescence)
Immunogen
Keratin-enriched preparation from human epidermoid carcinoma cell line A431
Region
PAN REACTIVE
Preparation and Storage
IgG concentration 1.0mg/ml

Application Data

(Published customer image:Mouse anti human cytokeratin (Pan reactive) antibody, clone C-11 used to label cytokeratins in bovine recto-anal junction tissue sections by immunofluorescence.Image caption:Immunofluorescent images of tissue sections from RAJ-IVOC adherence assay trial 1. The RAJ-IVOC were inoculated with either O157 (106 CFU inoculum as shown in parenthesis) or not inoculated (no bacteria) and incubated at 39 °C for 3 or 24 h. Tissue sections of the RAJ-IVOC were then stained with immunofluorescent antibodies targeting the RAJ cells’ cytokeratins and O157, and images were recorded at 100× magnification. The adherent bacteria (shown with arrows), RAJ cells’ cytokeratins, and the nuclei have green, orange–red, and blue fluorescence, respectively. The squamous (S), junction (J, boxed), and columnar (C) regions of the RAJ are indicated along with a 100 um scale bar.)

product-image-AAA50486_AD10.jpg Application Data (Published customer image:Mouse anti human cytokeratin (Pan reactive) antibody, clone C-11 used to label cytokeratins in bovine recto-anal junction tissue sections by immunofluorescence.Image caption:Immunofluorescent images of tissue sections from RAJ-IVOC adherence assay trial 1. The RAJ-IVOC were inoculated with either O157 (106 CFU inoculum as shown in parenthesis) or not inoculated (no bacteria) and incubated at 39 °C for 3 or 24 h. Tissue sections of the RAJ-IVOC were then stained with immunofluorescent antibodies targeting the RAJ cells’ cytokeratins and O157, and images were recorded at 100× magnification. The adherent bacteria (shown with arrows), RAJ cells’ cytokeratins, and the nuclei have green, orange–red, and blue fluorescence, respectively. The squamous (S), junction (J, boxed), and columnar (C) regions of the RAJ are indicated along with a 100 um scale bar.)

Application Data

(Published customer image:Mouse anti pan Cytokeratin antibody, clone C-11 (AAA50486) used to label cytokeratins in bovine cells by immunofluorescence.Image caption:Adherence patterns of EDL933 strains and mutants, obtained from sources A, B, and C, on RSE cells. The immunofluorescent stained slides are shown at 40x magnification. O157 have green fluorescence; RSE cells’ cytokeratins have orange-red fluorescence, and nuclei have blue fluorescence.)

product-image-AAA50486_AD11.jpg Application Data (Published customer image:Mouse anti pan Cytokeratin antibody, clone C-11 (AAA50486) used to label cytokeratins in bovine cells by immunofluorescence.Image caption:Adherence patterns of EDL933 strains and mutants, obtained from sources A, B, and C, on RSE cells. The immunofluorescent stained slides are shown at 40x magnification. O157 have green fluorescence; RSE cells’ cytokeratins have orange-red fluorescence, and nuclei have blue fluorescence.)

IF (Immunofluorescence)

(Immunofluorescence staining of MCF-7 cells spiked in blood samples and captured using the Genesis System and CelSelect SlidesTM Technology. Leukocytes (red) are identified using Mouse anti Human CD45 antibody and detected using a DyLight 550 conjugated Human anti Mouse IgG2a secondary antibody (HCA310D550). Immunolabeling of MCF-7 cells (green) was performed using Mouse anti Human Cytokeratin (Pan reactive) antibody (AAA50486) and detected using a FITC conjugated Goat anti Mouse IgG1 secondary antibody . Imaged using Nikon Ti2 at 20x magnification.)

product-image-AAA50486_IF13.png IF (Immunofluorescence) (Immunofluorescence staining of MCF-7 cells spiked in blood samples and captured using the Genesis System and CelSelect SlidesTM Technology. Leukocytes (red) are identified using Mouse anti Human CD45 antibody and detected using a DyLight 550 conjugated Human anti Mouse IgG2a secondary antibody (HCA310D550). Immunolabeling of MCF-7 cells (green) was performed using Mouse anti Human Cytokeratin (Pan reactive) antibody (AAA50486) and detected using a FITC conjugated Goat anti Mouse IgG1 secondary antibody . Imaged using Nikon Ti2 at 20x magnification.)

IF (Immunofluorescence)

(Immunofluorescence staining of MCF-7 cells spiked in blood samples and captured using the Genesis System and CelSelect SlidesTM Technology. Leukocytes (red) are identified using Mouse anti Human CD45 antibody and detected using a DyLight 550 conjugated Human anti Mouse IgG2a secondary antibody (HCA310D550). Immunolabeling of MCF-7 cells (green) was performed using Mouse anti Human Cytokeratin (Pan reactive) antibody (AAA50486) and detected using a FITC conjugated Goat anti Mouse IgG1 secondary antibody . Imaged using Nikon Ti2 at 20x magnification.)

product-image-AAA50486_IF15.jpg IF (Immunofluorescence) (Immunofluorescence staining of MCF-7 cells spiked in blood samples and captured using the Genesis System and CelSelect SlidesTM Technology. Leukocytes (red) are identified using Mouse anti Human CD45 antibody and detected using a DyLight 550 conjugated Human anti Mouse IgG2a secondary antibody (HCA310D550). Immunolabeling of MCF-7 cells (green) was performed using Mouse anti Human Cytokeratin (Pan reactive) antibody (AAA50486) and detected using a FITC conjugated Goat anti Mouse IgG1 secondary antibody . Imaged using Nikon Ti2 at 20x magnification.)
Related Product Information for anti-KRT6A antibody
Mouse anti human cytokeratin (Pan reactive) antibody, clone C-11 recognizes the human cytokeratins 4, 5, 6, 8, 10, 13 and 18.Cytokeratins, also known as keratins, form a subfamily of cytoplamsic proteins expressed in epithelial cells where they form intermediate filaments responsible for providing cells resistance to mechanical stress. Cytokeratins are characterized by their remarkable biochemical diversity, with epithelial tissues expressing at least 20 different cytokeratins (cytokeratin 1 to 20) divided into type I cytokeratins (acidic cytokeratins 9 to 20) and type II cytokeratins (basic to neutral cytokeratins 1 to 8) subfamilies. Cytokeratin expression profile is largely specific to particular organs and tissues and their development stage. That tissue-specific expression profile of cytokeratins is usually retained upon epithelial cell transformation and is therefore used in clinic to identify the cells from which carcinomas originated from. Mouse anti Human Cytokeratin (Pan reactive) antibody, clone C-11, has been validated for use on the Genesis Cell Isolation System with the CelSelect SlideTM technology.
Product Categories/Family for anti-KRT6A antibody

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Product Notes

The KRT6A (Catalog #AAA50486) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI HUMAN CYTOKERATIN (PAN REACTIVE) reacts with Human, Mammals and may cross-react with other species as described in the data sheet. AAA Biotech's CYTOKERATIN can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot), FCM/FACS (Flow Cytometry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the KRT6A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CYTOKERATIN, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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