IHC (Immunohistochemistry)
Mouse Cytokeratin HMW 34BE12 Monoclonal Antibody
Cytokeratin HMW 34BE12
Reactivity
Human, Dog, Cat, Monkey, Rabbit, Cattle, Horse
Synonyms
Cytokeratin HMW 34BE12, Antibody; Cytokeratin HMW 34BE12; anti-Cytokeratin HMW 34BE12 antibody
Host
Mouse
Reactivity
Human, Dog, Cat, Monkey, Rabbit, Cattle, Horse
Clonality
Monoclonal
Isotype
IgG1/K
Clone Number
34betaE12
Form/Format
Cytokeratin 34betaE12 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Application Notes
Recommended Immunohistochemical Protocol
Pretreatment
1. Cut and mount 3-4 micron formalin-fixed paraffin-embedded tissues on positive charged slides.
2. Air dry for 2 hours at 58° C.
3. Oeparaffinize, dehydrate and rehydrate tissues.
4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate or EDTA
5. Any of three heating methods may be used:
a. Electric Pressure Cooker
Place standoff rack at base of pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high, and incubate for 15 minutes. Open and immediately transfer slides to room temperature.
b. Water Bath Method
Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a water bath set at 95°-99° C.lncubate for 30-60 minutes.
c. Conventional Steamer Method
Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes.
6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.
7. Wash slides with IHC wash buffer or 01 water.
8. Continue IHC staining protocol.
Pretreatment
1. Cut and mount 3-4 micron formalin-fixed paraffin-embedded tissues on positive charged slides.
2. Air dry for 2 hours at 58° C.
3. Oeparaffinize, dehydrate and rehydrate tissues.
4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate or EDTA
5. Any of three heating methods may be used:
a. Electric Pressure Cooker
Place standoff rack at base of pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high, and incubate for 15 minutes. Open and immediately transfer slides to room temperature.
b. Water Bath Method
Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a water bath set at 95°-99° C.lncubate for 30-60 minutes.
c. Conventional Steamer Method
Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes.
6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.
7. Wash slides with IHC wash buffer or 01 water.
8. Continue IHC staining protocol.
Control
Prostate
Localization
Cytoplasmic
Reactivity Note
Paraffin, Frozen
Limitations
Cytokeratin 34Betae12 antibody, when used as directed, detects antigens that survive routine formalin fixation, tissue processing and sectioning. Users who deviate from recommended test procedures are responsible for interpretation and validaion of patien results.
Preparation and Storage
Store at 2 to 8 degree C in the dark.
For long-term storage of the concentrated antibody, it is recommended that aliquots of the antibody be frozen at -20 degree C in glycerol 50% (frost-free freezers are not recommended). Repeated freezing and thawing must be avoided. Dilute using an antibody diluent such as ImmunoDector Protein Block/Antibody Diluent or ImmunoDNA Background Blocker.
For long-term storage of the concentrated antibody, it is recommended that aliquots of the antibody be frozen at -20 degree C in glycerol 50% (frost-free freezers are not recommended). Repeated freezing and thawing must be avoided. Dilute using an antibody diluent such as ImmunoDector Protein Block/Antibody Diluent or ImmunoDNA Background Blocker.
IHC (Immunohistochemistry)
IHC (Immunohistochemistry)
Dilution Information
Dilution Information
Immunohistochemical Protocol
Immunohistochemical Protocol
Related Product Information for anti-Cytokeratin HMW 34BE12 antibody
Cytokeratin 34betaE12 is a High Molecular Weight cytokeratin that reacts with all squamous and ductal epithelium and stains carcinomas. This antibody recognizes cytokeratins 1, 5, 10, and 14 that are found in complex epithe-lia. Cytokeratin 34betaE12 shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules or endometrial glands; there has been no reactivity with cells derived from simple epithelia. Nerve cells, glial cells and mesenchymal tissue such as blood vessels containing only non-keratin types of intermediate filaments are not labelled; however, reactivity with smooth-muscle cells has been occasionally observed. Mesenchymal Tumors, Lymphomas, Melanomas, Neural Tumors and Neuroendocrine Tumors are unreactive with this antibody. Cytokeratin 34betaE12 has been shown to be useful in distinguishing Prostatic Adenocarcinoma from Hyperplasia of the Prostate.
Similar Products
Product Notes
The Cytokeratin HMW 34BE12 (Catalog #AAA13557) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Cytokeratin HMW 34BE12 reacts with Human, Dog, Cat, Monkey, Rabbit, Cattle, Horse and may cross-react with other species as described in the data sheet. Recommended Immunohistochemical Protocol Pretreatment 1. Cut and mount 3-4 micron formalin-fixed paraffin-embedded tissues on positive charged slides. 2. Air dry for 2 hours at 58° C. 3. Oeparaffinize, dehydrate and rehydrate tissues. 4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate or EDTA 5. Any of three heating methods may be used: a. Electric Pressure Cooker Place standoff rack at base of pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high, and incubate for 15 minutes. Open and immediately transfer slides to room temperature. b. Water Bath Method Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a water bath set at 95°-99° C.lncubate for 30-60 minutes. c. Conventional Steamer Method Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes. 6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes. 7. Wash slides with IHC wash buffer or 01 water. 8. Continue IHC staining protocol. . Researchers should empirically determine the suitability of the Cytokeratin HMW 34BE12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytokeratin HMW 34BE12, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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