Mouse DAAM1 Monoclonal Antibody | anti-DAAM1 antibody

DAAM1 (N-terminal region) Antibody

Cat. Num. AAA71617

• Reactivity: Human, Rat, Mouse
• Applications: Immunocytochemistry, ELISA, Western Blot
• Synonyms: DAAM1, Antibody; DAAM1 (N-terminal region) Antibody; DAAM1 (N-terminal region); anti-DAAM1 antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 5D3
• Specificity: The antibody detects a 125kDa* protein corresponding to the apparent molecular mass of DAAM1 on SDS-PAGE immunoblots of human A431, K562, and mouse C2C12 cells.
• Form/Format: Mouse monoclonal antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-DAAM1 antibody: ICC (Immunocytochemistry), ELISA, WB (Western Blot)
• Immunogen: Clone 5D3 was generated from a recombinant protein that included amino acid residues 1 to 111 in human DAAM1. This sequence has less than 50% homology to the N-terminal region of DAAM2.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

ICC (Immunocytochemistry)

(Immunocytochemical labeling of DAAM1 in aldehyde-fixed and NP-40-permeabilized A431 cells. The cells were labeled with mouse monoclonal DAAM1 (N-terminal region) antibody, then the antibody was detected using appropriate secondary antibody conjugated to DyLight 594. The corresponding phase image is shown to the right.)

WB (Western Blot)

(Western blot analysis of DAAM1 expression in mouse C2C12 (lane 1), human A431 (lane 2), and K562 (lane 3) cell lysates. The blots were probed with mouse monoclonal DAAM1 (N-terminal region) antibody at 1:500.)

Related Product Information for anti-DAAM1 antibody

Formins include several families of proteins that regulate actin cytoskeletal dynamics via two conserved formin homology domains, FH1 and FH2. The FH1 region contains poly-proline stretches that promote interactions with profilin. The FH2 domain, located C-terminally to the FH1 domain, is highly conserved in formin proteins and possesses actin nucleation and polymerization activities. Through cooperation of FH1 and FH2, formins construct actin-based structures comprising linear, unbranched filaments that are used in stress fibers, actin cables, microspikes, and contractile rings. Dishevelled associated activator of morphogenesis proteins (DAAM1 and DAAM2) are formin family members involved in WNT signaling. DAAM1 is ubiquitously expressed and may be important for regulating actin dynamics in several cell types. DAAM1 can bind RhoGTPase and dishevelled in WNT signaling pathways, and interacts with the SH3 domains of cell signaling mediators, such as c-Src.

References

Aspenstrom, P. et al. (2006) Exp Cell Res. 312(12):2180.
Kida, Y. et al. (2004) Brain Res Dev Brain Res. 153(1):143.
Habas, R. et al. (2001) Cell 107(7):843.

NCBI and Uniprot Product Information

• NCBI GI #: 21071077
• NCBI GeneID: 23002
• NCBI Accession #: NP_055807.1
• NCBI GenBank Nucleotide #: NM_014992.2
• UniProt Accession #: Q9Y4D1; Q86U34; Q8N1Z8; Q8TB39
• Molecular Weight: 100,745 Da
• NCBI Official Full Name: disheveled-associated activator of morphogenesis 1 isoform 1
• NCBI Official Synonym Full Names: dishevelled associated activator of morphogenesis 1
• NCBI Official Symbol: DAAM1
• NCBI Protein Information: disheveled-associated activator of morphogenesis 1
• UniProt Protein Name: Disheveled-associated activator of morphogenesis 1
• UniProt Gene Name: DAAM1
• UniProt Synonym Gene Names: KIAA0666
• UniProt Entry Name: DAAM1_HUMAN