FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of K562 cells with DAP12 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Rabbit anti-Human DAP12 Monoclonal Antibody | anti-DAP12 antibody
DAP12 Antibody
Gene Names
TYROBP; DAP12; KARAP; PLOSL
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
ProA affinity purified
Synonyms
DAP12, Antibody; DAP12 Antibody; DAP 12 antibody; DAP12 antibody; DNAX activation protein 12 antibody; DNAX-activation protein 12 antibody; KAR-associated protein antibody; KARAP antibody; Killer activating receptor associated protein antibody; Killer-activating receptor-associated protein antibody; PLOSL antibody; TYOBP_HUMAN antibody; TYRO protein tyrosine kinase binding protein antibody; TYRO protein tyrosine kinase-binding protein antibody; TYROBP antibody; anti-DAP12 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
JG38-70
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-DAP12 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Synthetic peptide within human DAP12 aa 60-100.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of K562 cells with DAP12 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of K562 cells with DAP12 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-DAP12 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-DAP12 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of DAP12 on THP-1 cell using anti-DAP12 antibody at 1/500 dilution.)
WB (Western Blot)
(Western blot analysis of DAP12 on THP-1 cell using anti-DAP12 antibody at 1/500 dilution.)
Related Product Information for anti-DAP12 antibody
Natural killer (NK) cells are regulated by stimulatory and inhibitory signals from a variety of receptors. Three main receptor families are responsible for NK cells recognition of MHC I molecules, including Ly-49, CD94/NKG2 and KIR (killer-cell inhibitory receptor). DAP12 is a phosphoprotein that is involved in the activation of NK cells. This protein interacts with membrane glycoproteins of the KIR family, resulting in cellular activation. DAP12 also binds to CD94/NKG2C, an activating NK cell receptor belonging to the C-type lectin superfamily. Additional proteins that bind to DAP12 include Ly-49D and Ly-49H, which associate with DAP12 in the plasma membrane. Phosphorylated DAP12 binds to ZAP-70 and Syk, suggesting that the activation pathway may be similar to that of the T and B cell antigen receptors.
Product Categories/Family for anti-DAP12 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,043 Da
NCBI Official Full Name
TYRO protein tyrosine kinase-binding protein isoform 1
NCBI Official Synonym Full Names
TYRO protein tyrosine kinase binding protein
NCBI Official Symbol
TYROBP
NCBI Official Synonym Symbols
DAP12; KARAP; PLOSL
NCBI Protein Information
TYRO protein tyrosine kinase-binding protein; KAR-associated protein; DNAX-activation protein 12; killer-activating receptor-associated protein
UniProt Protein Name
TYRO protein tyrosine kinase-binding protein
UniProt Gene Name
TYROBP
UniProt Synonym Gene Names
DAP12; KARAP; KAR-associated protein
UniProt Entry Name
TYOBP_HUMAN
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Product Notes
The DAP12 tyrobp (Catalog #AAA312681) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DAP12 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DAP12 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the DAP12 tyrobp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DAP12, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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