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WB (Western Blot)
(Western Blot analysis of various cells using primary antibody at 1:10000 dilution. Secondary antibody)
Mouse DDDDK-Tag Monoclonal Antibody | anti-DDDDK-Tag antibody
DDDDK-Tag (binds to DYKDDDDK sequnence) Antibody (12M11)
Reactivity
Species independent
Applications
Immunoprecipitation, Immunofluorescence, Immunoprecipitation, ELISA, Western Blot
Purity
Protein A purified
Synonyms
DDDDK-Tag, Antibody; DDDDK-Tag (binds to DYKDDDDK sequnence) Antibody (12M11); anti-DDDDK-Tag antibody
Host
Mouse
Reactivity
Species independent
Clonality
Monoclonal
Isotype
Mouse VH IgG1 and VL Kappa
Clone Number
12M11
Specificity
This recombinant antibody can highly specifically recognize the DYKDDDDK tags of C-terminal and N-terminal of recombinant protein. Variable region gene of this antibody comes from the mouse gene library immunized with DDDK peptide. Constant region of this antibody is mouse IgG1 and mouse kappa.
Purity/Purification
Protein A purified
Form/Format
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Applicable Applications for anti-DDDDK-Tag antibody
IP (CoImmunoprecipitation), IF (Immunofluorescence), IP (Immunoprecipitation), ELISA, WB (Western Blot)
Source
Mouse VH IgG1 and VL Kappa was expressed from 293F cells
Immunogen
Synthetic Peptide of DYKDDDDK-Tag
Modification
Unmodified/Total
Preparation and Storage
Store at -20 degree C/1 year
WB (Western Blot)
(Western Blot analysis of various cells using primary antibody at 1:10000 dilution. Secondary antibody)
WB (Western Blot)
(Western Blot analysis of various cells using primary antibody at 1:10000 dilution. Secondary antibody)
Application Data
(Diluted at 1:10000)
Application Data
(Diluted at 1:10000)
WB (Western Blot)
(Western Blot analysis of 293F cells transfected or non-tansfected DDDK-tag expression vector by primary antibody at 1:10000 dilution. Lane 1 3 5: 293F cell lysis. Lane 2 4 6: tansfected DDDK-tag expression vector. Secondary antibody)
WB (Western Blot)
(Western Blot analysis of 293F cells transfected or non-tansfected DDDK-tag expression vector by primary antibody at 1:10000 dilution. Lane 1 3 5: 293F cell lysis. Lane 2 4 6: tansfected DDDK-tag expression vector. Secondary antibody)
IF (Immunofluorescence)
(Immunofluorescence analysis of 293T transfected DYKDDDDK and GFP tag protein. 1, DYKDDDDK primary Antibody(red AAA320531) was diluted at 1:1000(4 degree C overnight), GFP primary Antibody(green C40014) was diluted at 1:1000(4 degree C overnight),)
IF (Immunofluorescence)
(Immunofluorescence analysis of 293T transfected DYKDDDDK and GFP tag protein. 1, DYKDDDDK primary Antibody(red AAA320531) was diluted at 1:1000(4 degree C overnight), GFP primary Antibody(green C40014) was diluted at 1:1000(4 degree C overnight),)
Product Categories/Family for anti-DDDDK-Tag antibody
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Product Notes
The DDDDK-Tag (Catalog #AAA320531) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The DDDDK-Tag (binds to DYKDDDDK sequnence) Antibody (12M11) reacts with Species independent and may cross-react with other species as described in the data sheet. AAA Biotech's DDDDK-Tag can be used in a range of immunoassay formats including, but not limited to, IP (CoImmunoprecipitation), IF (Immunofluorescence), IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the DDDDK-Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DDDDK-Tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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