IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300ug extracts of 293T-SERPINB1-Flag(N) cells using 3ug DDDDK-Tag antibody (AAA283345 1:100). Western blot was performed from the immunoprecipitate using DDDDK-Tag antibody (AE092) at a dilition of 1:5000.)
Rabbit anti-Human DDDDK-Tag Monoclonal Antibody | anti-DDDDK antibody
Rabbit anti DDDDK-Tag Rabbit PolymAb
Reactivity
Human
Applications
ELISA, Immunoprecipitation, Western Blot
Purity
Affinity purification
Synonyms
DDDDK-Tag, Antibody; Rabbit anti DDDDK-Tag Rabbit PolymAb; DDDDK;DDDDK tag;DDDDK-tag; anti-DDDDK antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Applicable Applications for anti-DDDDK antibody
ELISA, IP (Immunoprecipitation), WB (Western Blot)
Cross Reactivity
Species independent
Immunogen
A synthetic peptide corresponding to DDDDK-Tag.
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300ug extracts of 293T-SERPINB1-Flag(N) cells using 3ug DDDDK-Tag antibody (AAA283345 1:100). Western blot was performed from the immunoprecipitate using DDDDK-Tag antibody (AE092) at a dilition of 1:5000.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300ug extracts of 293T-SERPINB1-Flag(N) cells using 3ug DDDDK-Tag antibody (AAA283345 1:100). Western blot was performed from the immunoprecipitate using DDDDK-Tag antibody (AE092) at a dilition of 1:5000.)
WB (Western Blot)
(Western blot analysis of lysates from wild type (WT) and 293T-Renin-Flag(C) cells transfected with DDDDK-Tag using DDDDK-Tag Rabbit PolymAb® (AAA283345) at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020)Exposure time: 30s.)
WB (Western Blot)
(Western blot analysis of lysates from wild type (WT) and 293T-Renin-Flag(C) cells transfected with DDDDK-Tag using DDDDK-Tag Rabbit PolymAb® (AAA283345) at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020)Exposure time: 30s.)
Related Product Information for anti-DDDDK antibody
DYKDDDDK-tag, or DYKDDDDK octapeptide, or DYKDDDDK epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.A DYKDDDDK-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a DYKDDDDK-tag to a protein allows the protein to be studied with an antibody against the DYKDDDDK sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.
Product Categories/Family for anti-DDDDK antibody
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Product Notes
The DDDDK (Catalog #AAA283345) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti DDDDK-Tag Rabbit PolymAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DDDDK-Tag can be used in a range of immunoassay formats including, but not limited to, ELISA, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the DDDDK for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DDDDK-Tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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