Mouse DMC1 Monoclonal Antibody | anti-DMC1 antibody

DMC1

Cat. Num. AAA214893

• Gene Names: DMC1; DMC1H; LIM15; dJ199H16.1
• Reactivity: Human, Mouse, Rat, Cow
• Applications: Immunohistochemistry, Western Blot, Immunofluorescence
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: DMC1, Antibody; DMC1; Disrupted meiotic cDNA 1 homolog; dJ199H16.1; DMC1 dosage suppressor of mck1 homolog; DMC1H; DNA meiotic recombinase 1; HsLim15; LIM15; Meiotic recombination protein DMC1/LIM15 homolog; MGC150472; MGC150473; anti-DMC1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Cow
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: 2H12/4
• Specificity: DNA repair proteins are necessary for the maintenance of chromosome integrity and are involved in the elimination of premutagenic lesions from DNA. The DNA repair proteins Rad51 and Rad52 are key components of the doublestrand-break repair (DSBR) pathway. Rad51 is essential for mitotic and meiotic recombination, and its mutation in yeast and mammalian cells results in chromosome loss. Overexpression of Rad52 confers resistance to ionizing radiation and induces homologous intrachromosomal recombination. Rad52 is thought to be involved in an early stage of Rad51-mediated recombination. Additional proteins involved in the pathway include Nibrin and Dmc1. Nibrin, which complexes with Mre11 and Rad50, is absent in Nijemegen breakage syndrome (NBS) patients. Dmc1 is specifically involved in meiotic recombination. An alternative spliced form of Dmc1, designated Dmc1-D, is deleted for a region between the two motifs involved in nucleotide binding. The alternatively spliced Dmc1-D transcript is detected in both male and female germ cells, indicating that the encoded protein may have a role in mammalian genetic recombination in meiosis.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 340

• Applicable Applications for anti-DMC1 antibody: IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence)
• Positive Control: HeLa cells. Cervix or prostate.
• Immunogen: Recombinant full-length human DMC1 protein.
• Cellular Localization: Nucleus. Chromosome.
• Hu-chromosome Location: 22q13.1
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

IHC (Immunohistochemisry)

(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with CLEC9A Mouse Monoclonal Antibody (2H12/4).)

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with CLEC9A Mouse Monoclonal Antibody (2H12/4).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with CLEC9A Mouse Monoclonal Antibody (2H12/4).)

References

Morita, T., et al. 1993. Proc. Natl. Acad. Sci. USA 90: 6577-6580.

NCBI and Uniprot Product Information

• NCBI GI #: 23238219
• NCBI GeneID: 11144
• NCBI Accession #: NP_008999.2
• NCBI GenBank Nucleotide #: NP_008999.2
• UniProt Accession #: Q14565
• Molecular Weight: 37kDa
• NCBI Official Full Name: meiotic recombination protein DMC1/LIM15 homolog isoform 1
• NCBI Official Synonym Full Names: DNA meiotic recombinase 1
• NCBI Official Symbol: DMC1
• NCBI Official Synonym Symbols: DMC1H; LIM15; dJ199H16.1
• NCBI Protein Information: meiotic recombination protein DMC1/LIM15 homolog
• UniProt Protein Name: Meiotic recombination protein DMC1/LIM15 homolog
• UniProt Gene Name: DMC1
• UniProt Synonym Gene Names: DMC1H; LIM15