FCM/FACS (Flow Cytometry)
(DMC1 Antibody (N-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human DMC1 Polyclonal Antibody | anti-DMC1 antibody
DMC1 Antibody (N-term)
Gene Names
DMC1; DMC1H; LIM15; dJ199H16.1
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
DMC1, Antibody; DMC1 Antibody (N-term); Meiotic recombination protein DMC1/LIM15 homolog; DMC1; DMC1H; LIM15; anti-DMC1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This DMC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human DMC1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
1-30
Sequence Length
285
Applicable Applications for anti-DMC1 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(DMC1 Antibody (N-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(DMC1 Antibody (N-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with DMC1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with DMC1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of DMC1 Antibody (N-term) in MDA-MB435 cell line lysates (35ug/lane). DMC1 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of DMC1 Antibody (N-term) in MDA-MB435 cell line lysates (35ug/lane). DMC1 (arrow) was detected using the purified Pab.)
Related Product Information for anti-DMC1 antibody
DMC1 is essential for meiotic homologous recombination. Genetic recombination in meiosis plays an important role in generating diversity of genetic information. The product of this protein is structurally and evolutionary related to the products of the yeast RAD51 and E. coli RecA genes.
References
Volodin,A.A. J. Biol. Chem. 284 (3), 1495-1504 (2009)
Hikiba,J.FEBS J. 276 (2), 457-465 (2009)
Hikiba,J. Nucleic Acids Res. 36 (12), 4181-4190 (2008)
Hikiba,J.FEBS J. 276 (2), 457-465 (2009)
Hikiba,J. Nucleic Acids Res. 36 (12), 4181-4190 (2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37681
NCBI Official Full Name
meiotic recombination protein DMC1/LIM15 homolog isoform 1
NCBI Official Synonym Full Names
DNA meiotic recombinase 1
NCBI Official Symbol
DMC1
NCBI Official Synonym Symbols
DMC1H; LIM15; dJ199H16.1
NCBI Protein Information
meiotic recombination protein DMC1/LIM15 homolog
UniProt Protein Name
Meiotic recombination protein DMC1/LIM15 homolog
UniProt Gene Name
DMC1
UniProt Synonym Gene Names
DMC1H; LIM15
UniProt Entry Name
DMC1_HUMAN
Similar Products
Product Notes
The DMC1 dmc1 (Catalog #AAA286824) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-30. The DMC1 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DMC1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the DMC1 dmc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DMC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.