<
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Breast cancer using DNA-PKcs mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).)
Mouse anti-Human DNA-PKcs Monoclonal Antibody | anti-DNA-PKcs antibody
DNA-PKcs Mouse mAb
Gene Names
PRKDC; HYRC; p350; DNAPK; DNPK1; HYRC1; XRCC7; DNA-PKcs
Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, Western Blot
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Synonyms
DNA-PKcs, Antibody; DNA-PKcs Mouse mAb; DNA dependent protein kinase catalytic subuni; DNA PKcs; DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNAPK; DNPK1; hyper radiosensitivity of murine scid mutation, complementing 1; HYRC; HYRC1; p350; p460; PRKDC; PRKDC_HUMAN; protein kinase, DNA activated, catalytic polypeptide; XRCC7.; anti-DNA-PKcs antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Specificity
This antibody detects endogenous levels of DNA-PKcs and does not cross-react with related proteins.
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-DNA-PKcs antibody
IHC (Immunohistochemistry), ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Purified recombinant human DNA-PKcs protein fragments expressed in E coli
Modification
Unmodified/Total
Preparation and Storage
Store at -20 degree C/1 year
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Breast cancer using DNA-PKcs mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Breast cancer using DNA-PKcs mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).)
ICC (Immunocytochemistry)
(Immunocytochemistry stain of Hela using DNA-PKcs mouse mAb (1:100).)
ICC (Immunocytochemistry)
(Immunocytochemistry stain of Hela using DNA-PKcs mouse mAb (1:100).)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of Hela cell lysate using DNA-PKcs mouse mAb.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of Hela cell lysate using DNA-PKcs mouse mAb.)
WB (Western Blot)
(Western blot detection of DNA-PKcs in Hela.Molt-4.A549 and HCT116 cell lysates using DNA-PKcs mouse mAb (1:1000 diluted).Predicted band size:450KDa.Observed band size:450KDa.)
WB (Western Blot)
(Western blot detection of DNA-PKcs in Hela.Molt-4.A549 and HCT116 cell lysates using DNA-PKcs mouse mAb (1:1000 diluted).Predicted band size:450KDa.Observed band size:450KDa.)
WB (Western Blot)
(Western blot detection of DNA-PKcs in Hela and K562 cell lysates using DNA-PKcs mouse mAb (1:1000 diluted).Predicted band size:450KDa.Observed band size:450KDa.)
WB (Western Blot)
(Western blot detection of DNA-PKcs in Hela and K562 cell lysates using DNA-PKcs mouse mAb (1:1000 diluted).Predicted band size:450KDa.Observed band size:450KDa.)
Related Product Information for anti-DNA-PKcs antibody
This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.[provided by RefSeq, Jul 2010]
Product Categories/Family for anti-DNA-PKcs antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
469,089 Da
NCBI Official Full Name
DNA-dependent protein kinase catalytic subunit isoform 2
NCBI Official Synonym Full Names
protein kinase, DNA-activated, catalytic polypeptide
NCBI Official Symbol
PRKDC
NCBI Official Synonym Symbols
HYRC; p350; DNAPK; DNPK1; HYRC1; XRCC7; DNA-PKcs
NCBI Protein Information
DNA-dependent protein kinase catalytic subunit; p460; DNA-PK catalytic subunit; hyper-radiosensitivity of murine scid mutation, complementing 1
UniProt Protein Name
DNA-dependent protein kinase catalytic subunit
UniProt Gene Name
PRKDC
UniProt Synonym Gene Names
HYRC; HYRC1; DNA-PK catalytic subunit; DNA-PKcs
UniProt Entry Name
PRKDC_HUMAN
Similar Products
Product Notes
The DNA-PKcs prkdc (Catalog #AAA320345) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The DNA-PKcs Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNA-PKcs can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the DNA-PKcs prkdc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNA-PKcs, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.