WB (Western Blot)
(Detection of human DNA-PKcs by western blot and immunoprecipitation. Samples: Whole cell lysate (15 and 50 ug for WB; 1 mg for IP) from HeLa cells. Antibodies: Affinity purified rabbit anti-DNA-PKcs antibody BL2404 (Cat. No. AAA210996) used at 0.1 ug/ml for WB (A and B) and at 3 ug/mg lysate for IP (B). DNA-PKcs was also immunoprecipitated using rabbit anti-DNA-PKcs antibodies BL2402 (Cat. No. BL2403 (Cat. No. and BL2406 (Cat. No. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human DNA-PKcs Polyclonal Antibody | anti-DNA-PKcs antibody
Rabbit anti-DNA-PKcs Antibody, Affinity Purified
Gene Names
PRKDC; HYRC; p350; DNAPK; DNPK1; HYRC1; IMD26; XRCC7; DNA-PKcs
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
DNA-PKcs, Antibody; Rabbit anti-DNA-PKcs Antibody, Affinity Purified; hyper-radiosensitivity of murine scid mutation, complementing 1; IMD26; XRCC7; protein kinase, DNA-activated, catalytic polypeptide; p350; HYRC1; HYRC; DNA-PKcs; DNA-PKC; DNA-dependent protein kinase catalytic subunit; DNAPKc; p460; DNA-PK catalytic subunit; DNAPK; DNPK1; protein kinase, DNA-activated, catalytic subunit; anti-DNA-PKcs antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-DNA-PKcs antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 2100 and 2150
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human DNA-PKcs by western blot and immunoprecipitation. Samples: Whole cell lysate (15 and 50 ug for WB; 1 mg for IP) from HeLa cells. Antibodies: Affinity purified rabbit anti-DNA-PKcs antibody BL2404 (Cat. No. AAA210996) used at 0.1 ug/ml for WB (A and B) and at 3 ug/mg lysate for IP (B). DNA-PKcs was also immunoprecipitated using rabbit anti-DNA-PKcs antibodies BL2402 (Cat. No. BL2403 (Cat. No. and BL2406 (Cat. No. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human DNA-PKcs by western blot and immunoprecipitation. Samples: Whole cell lysate (15 and 50 ug for WB; 1 mg for IP) from HeLa cells. Antibodies: Affinity purified rabbit anti-DNA-PKcs antibody BL2404 (Cat. No. AAA210996) used at 0.1 ug/ml for WB (A and B) and at 3 ug/mg lysate for IP (B). DNA-PKcs was also immunoprecipitated using rabbit anti-DNA-PKcs antibodies BL2402 (Cat. No. BL2403 (Cat. No. and BL2406 (Cat. No. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IHC (Immunohistochemistry)
(Detection of human DNA-PKcs by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-DNA-PKcs (Cat. No. AAA210996) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human DNA-PKcs by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-DNA-PKcs (Cat. No. AAA210996) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-DNA-PKcs antibody
DNA-PKcs is the catalytic subunit of a DNA-dependent serine-threonine protein kinase (DNA-PK). DNA-PK is a heterotrimer comprised of DNA-PKcs and the Ku709/Ku86 dimer. DNA-PK is involved in DNA damage repair and V(D)J recombination. DNA-PK phosphorylates Artemis, a protein required for nonhomologous end-joining and is necessary for the loading of Artemis on damaged DNA.
Product Categories/Family for anti-DNA-PKcs antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
465,501 Da
NCBI Official Full Name
DNA-dependent protein kinase catalytic subunit isoform 1
NCBI Official Synonym Full Names
protein kinase, DNA-activated, catalytic polypeptide
NCBI Official Symbol
PRKDC
NCBI Official Synonym Symbols
HYRC; p350; DNAPK; DNPK1; HYRC1; IMD26; XRCC7; DNA-PKcs
NCBI Protein Information
DNA-dependent protein kinase catalytic subunit; p460; DNA-PK catalytic subunit; hyper-radiosensitivity of murine scid mutation, complementing 1
UniProt Protein Name
DNA-dependent protein kinase catalytic subunit
UniProt Gene Name
PRKDC
UniProt Synonym Gene Names
HYRC; HYRC1; DNA-PK catalytic subunit; DNA-PKcs
UniProt Entry Name
PRKDC_HUMAN
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Product Notes
The DNA-PKcs prkdc (Catalog #AAA210996) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-DNA-PKcs Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNA-PKcs can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the DNA-PKcs prkdc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNA-PKcs, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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