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IHC (Immunohistchemistry)
(Formalin-paraffin human Placenta stained with E-Cadherin MAb (CDH1/1525).)
Mouse E-Cadherin/CD324 Monoclonal Antibody | anti-CD324 antibody
E-Cadherin/CD324 (Intercellular Junction Marker)
Gene Names
CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324
Reactivity
Human.Does not react with Mouse and Rat. Others not known.
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
Synonyms
E-Cadherin/CD324, Antibody; E-Cadherin/CD324 (Intercellular Junction Marker); Arc 1; cadherin 1 type 1 E-cadherin; Cadherin1; CAM 120/80; CD324; CDH1; CDHE; E-Cad/CTF3; E-cadherin; ECAD; Epithelial cadherin; epithelial calcium dependent adhesion protein; Liver cell adhesion molecule (LCAM); Uvomorulin (UVO); anti-CD324 antibody
Host
Mouse
Reactivity
Human.
Does not react with Mouse and Rat. Others not known.
Does not react with Mouse and Rat. Others not known.
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
CDH1/1525
Specificity
Recognizes a protein of 120-80kDa, identified as E-cadherin. Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as beta-catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin +) and lobular (E-cadherin -) breast carcinomas. It may also help of mesothelioma.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
821
Applicable Applications for anti-CD324 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
Application Notes
FC/FACS: 0.5-1ug/million cells
IF: 0.5-1ug/ml
WB: 0.5-1ug/ml
IHC-F: 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
IF: 0.5-1ug/ml
WB: 0.5-1ug/ml
IHC-F: 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Immunogen
Recombinant human full-length E-Cadherin protein
Cellular Localization
Cell Surface
Hu-chromosome Location
16q22.1
Positive Control
LS174T, Raji, HT29, SK-BR3 cells. Prostate or Colon carcinomas.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
IHC (Immunohistchemistry)
(Formalin-paraffin human Placenta stained with E-Cadherin MAb (CDH1/1525).)
IHC (Immunohistchemistry)
(Formalin-paraffin human Placenta stained with E-Cadherin MAb (CDH1/1525).)
IHC (Immunohistochemistry)
(Formalin-paraffin human Prostate Carcinoma stained with E-Cadherin MAb (CDH1/1525).)
IHC (Immunohistochemistry)
(Formalin-paraffin human Prostate Carcinoma stained with E-Cadherin MAb (CDH1/1525).)
IHC (Immunohistochemistry)
(Formalin-paraffin human Colon Carcinoma stained with E-Cadherin MAb (CDH1/1525).)
IHC (Immunohistochemistry)
(Formalin-paraffin human Colon Carcinoma stained with E-Cadherin MAb (CDH1/1525).)
WB (Western Blot)
(Western Blot Analysis (A) Recombinant Protein (B) human Stomach lysate Using E-Cadherin Monoclonal Antibody (CDH1/1525).)
WB (Western Blot)
(Western Blot Analysis (A) Recombinant Protein (B) human Stomach lysate Using E-Cadherin Monoclonal Antibody (CDH1/1525).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Skin stained with E-Cadherin Monoclonal Antibody (CDH1/1525).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Skin stained with E-Cadherin Monoclonal Antibody (CDH1/1525).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with E-Cadherin Monoclonal Antibody (CDH1/1525).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with E-Cadherin Monoclonal Antibody (CDH1/1525).)
References
Umbas, R., et al. 1992. Expression of the cellular adhesion molecule E-cadherin is reduced or absent in high-grade prostate cancer. Cancer Res. 52: 5104-5109.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
120-80kDa (Mature); 135kDa (Precursor)
NCBI Official Full Name
cadherin-1 isoform 2
NCBI Official Synonym Full Names
cadherin 1
NCBI Official Symbol
CDH1
NCBI Official Synonym Symbols
UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324
NCBI Protein Information
cadherin-1
UniProt Protein Name
Cadherin-1
UniProt Gene Name
CDH1
UniProt Synonym Gene Names
CDHE; UVO; E-cadherin
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Product Notes
The CD324 cdh1 (Catalog #AAA23896) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The E-Cadherin/CD324 (Intercellular Junction Marker) reacts with Human. Does not react with Mouse and Rat. Others not known. and may cross-react with other species as described in the data sheet. AAA Biotech's E-Cadherin/CD324 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin. FC/FACS: 0.5-1ug/million cells IF: 0.5-1ug/ml WB: 0.5-1ug/ml IHC-F: 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Researchers should empirically determine the suitability of the CD324 cdh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "E-Cadherin/CD324, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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