Application Data
Mouse E-Cadherin (Cytoplasmic) Monoclonal Antibody | anti-CDH1 antibody
E-Cadherin (Cytoplasmic)
Gene Names
CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; CD324
Reactivity
Human, Rat, Mouse
Applications
Immunoprecipitation, Immunocytochemistry, Western Blot, ELISA
Purity
Protein A Purified
Synonyms
E-Cadherin (Cytoplasmic), Antibody; E-Cadherin (Cytoplasmic); Uvomorulin; Cadherin-1; anti-CDH1 antibody
Host
Mouse
Reactivity
Human, Rat, Mouse
Clonality
Monoclonal
Isotype
IgG1
Specificity
This E-cadherin antibody detects a 120 kDa* protein in human A431 cells, and does not cross-react with Vecadherin or N-cadherin.
Purity/Purification
Protein A Purified
Form/Format
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100ml phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
Sequence Length
882
Applicable Applications for anti-CDH1 antibody
IP (Immunoprecipitation), ICC (Immunocytochemistry), WB (Western Blot), ELISA
Immunogen
Clone (M168) was generated from a mouse recombinant E-Cadherin protein containing amino acids in the C-terminal region. This sequence is
highly conserved in human and rat E-cadherin.
Preparation and Storage
Store at -20 degree C. Do not aliquot. Stable for 1 year.
Application Data
Application Data
Related Product Information for anti-CDH1 antibody
Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the protein p120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. N-cadherin is phosphorylated by c-Src at Tyr-820, Tyr-853, Tyr-860, Tyr-884, and Tyr -886. Phosphorylation of Tyr-860 can disrupt cadherin binding to bcatenin. Since many of these tyrosine sites are conserved in the cadherin family, phosphorylation of these sites may be critical for cadherin function.
References
• Takeichi, M. (1988) Development 102:639
• Xu, Y. et al. (1997) J. Biol. Chem. 272(21):13463
• Qi, J. et al. (2006) Mol. Biol. Cell 17(3):1261
• Xu, Y. et al. (1997) J. Biol. Chem. 272(21):13463
• Qi, J. et al. (2006) Mol. Biol. Cell 17(3):1261
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
97,456 Da
NCBI Official Full Name
Cadherin-1
NCBI Official Synonym Full Names
cadherin 1, type 1, E-cadherin (epithelial)
NCBI Official Symbol
CDH1
NCBI Official Synonym Symbols
UVO; CDHE; ECAD; LCAM; Arc-1; CD324
NCBI Protein Information
cadherin-1; CAM 120/80; E-Cadherin; uvomorulin; cell-CAM 120/80; OTTHUMP00000174868; epithelial cadherin; cadherin 1, E-cadherin (epithelial); calcium-dependent adhesion protein, epithelial
UniProt Protein Name
Cadherin-1
UniProt Gene Name
CDH1
UniProt Synonym Gene Names
CDHE; UVO
UniProt Entry Name
CADH1_HUMAN
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Product Notes
The CDH1 cdh1 (Catalog #AAA71504) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The E-Cadherin (Cytoplasmic) reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's E-Cadherin (Cytoplasmic) can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the CDH1 cdh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "E-Cadherin (Cytoplasmic), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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