WB (Western Blot)
(A431 cells were subjected to SDS PAGE followed by western blot with AAA249059 (E-cadherin antibody) at dilution of 1:3000)
Mouse anti-Human ECAD Monoclonal Antibody | anti-ECAD antibody
Anti-E-cadherin Antibody
Gene Names
CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324
Reactivity
Human
Applications
Immunofluorescence, Western Blot, Immunohistochemistry, ELISA
Purity
>=95% as determined by SDS-PAGEProtein A+G purified
Synonyms
ECAD, Antibody; Anti-E-cadherin Antibody; Arc 1; Cadherin 1; CD324; CDH1; CDHE; E cadherin; ECAD; E-cadherin; LCAM; UVO; Uvomorulin; anti-ECAD antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
3G10
Purity/Purification
>=95% as determined by SDS-PAGE
Protein A+G purified
Protein A+G purified
Form/Format
Liquid; PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Sequence Length
882
Applicable Applications for anti-ECAD antibody
IF (Immunofluorescence), WB (Western Blot), IHC (Immunohistochemistry), ELISA
Immunogen
Cadherin-1, type 1, E-Cadherin-(epithelial)
Preparation and Storage
Store at -20 degree C for 24 months. Avoid repeated freeze/thaw cycles.
WB (Western Blot)
(A431 cells were subjected to SDS PAGE followed by western blot with AAA249059 (E-cadherin antibody) at dilution of 1:3000)
WB (Western Blot)
(A431 cells were subjected to SDS PAGE followed by western blot with AAA249059 (E-cadherin antibody) at dilution of 1:3000)
IF (Immunofluorescence)
(Immunofluorescent analysis of A431 cells using AAA249059 (E-cadherin Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L))
IF (Immunofluorescence)
(Immunofluorescent analysis of A431 cells using AAA249059 (E-cadherin Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L))
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using AAA249059 (E-cadherin Antibody) at dilution of 1:1000)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using AAA249059 (E-cadherin Antibody) at dilution of 1:1000)
Related Product Information for anti-ECAD antibody
This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16.
Product Categories/Family for anti-ECAD antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed MW: 130kDa
NCBI Official Full Name
cadherin-1 isoform 1 preproprotein
NCBI Official Synonym Full Names
cadherin 1
NCBI Official Symbol
CDH1
NCBI Official Synonym Symbols
UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324
NCBI Protein Information
cadherin-1
UniProt Protein Name
Cadherin-1
UniProt Gene Name
CDH1
UniProt Synonym Gene Names
CDHE; UVO; E-cadherin
UniProt Entry Name
CADH1_HUMAN
Similar Products
Product Notes
The ECAD cdh1 (Catalog #AAA249059) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-E-cadherin Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ECAD can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the ECAD cdh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ECAD, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.