Application Data
(Fig.2: CGE analysis of purified BGC-6F6 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 mug of purified BGC-6F6 antibody. Proteins were separated by CGE (capillary gel electrophoresis, Agilent 2100 Bioanalyzer). Internal control bands (240 kDa / 7 kDa / 4,5 kDa).)
Rat Ectodysplasin A Receptor (EDAR) Monoclonal Antibody | anti-EDAR antibody
Rat anti-human Ectodysplasin A Receptor (EDAR) monoclonal antibody
Gene Names
EDAR; DL; ED3; ED5; ED1R; EDA3; HRM1; EDA1R; ECTD10A; ECTD10B; EDA-A1R
Applications
ELISA, ELISA, Flow Cytometry, Functional Assay
Purity
Protein G
Synonyms
Ectodysplasin A Receptor (EDAR), Antibody; Rat anti-human Ectodysplasin A Receptor (EDAR) monoclonal antibody; anti-EDAR antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG2b/kappa
Clone Number
BGC-6F6
Specificity
anti-human EDAR
Purity/Purification
Protein G
Sequence Length
448
Applicable Applications for anti-EDAR antibody
ELISA, ELISA, FCM/FACS (Flow Cytometry)
Buffer
Phosphate buffered saline, pH 7.2
Immunogen
Genetic immunisation with cDNA encoding the full-length human EDAR
Selection
Based on recognition of the full-length native protein expressed on viable, transfected mammalian cells
Preparation and Storage
Short term: 2 degree C - 8 degree C
Long term: -20 degree C (avoid repeated freezing and thawing)
Long term: -20 degree C (avoid repeated freezing and thawing)
Application Data
(Fig.2: CGE analysis of purified BGC-6F6 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 mug of purified BGC-6F6 antibody. Proteins were separated by CGE (capillary gel electrophoresis, Agilent 2100 Bioanalyzer). Internal control bands (240 kDa / 7 kDa / 4,5 kDa).)
Application Data
(Fig.2: CGE analysis of purified BGC-6F6 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 mug of purified BGC-6F6 antibody. Proteins were separated by CGE (capillary gel electrophoresis, Agilent 2100 Bioanalyzer). Internal control bands (240 kDa / 7 kDa / 4,5 kDa).)
FCM/FACS (Flow Cytometry)
(Fig.1: GM-1008. BOSC23 cells were transiently transfected with an expression vector encoding either full length human EDAR (red filled curve), or an irrelevant protein (control transfectant, black curve). Binding of BGC-6F6 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with human EDAR transfected cells.)
FCM/FACS (Flow Cytometry)
(Fig.1: GM-1008. BOSC23 cells were transiently transfected with an expression vector encoding either full length human EDAR (red filled curve), or an irrelevant protein (control transfectant, black curve). Binding of BGC-6F6 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with human EDAR transfected cells.)
Related Product Information for anti-EDAR antibody
Ectodysplasin A receptor (EDAR) is a protein that is encoded by the EDAR gene in humans. EDAR is a cell surface receptor for ectodysplasin A. which plays an important role in the development of ectodermal tissues such as the skin (1). It is structurally related to members of the TNF receptor superfamily. EDAR and other genes provide instructions for making proteins that work together during embryonic development. These proteins form part of a signaling pathway that is critical for the interaction between two cell layers, the ectoderm and the mesoderm. In the early embryo, these cell layers form the basis for many of the body's organs and tissues. Ectoderm-mesoderm interactions are essential for the proper formation of several structures that arise from the ectoderm, including the skin, hair, nails, teeth, and sweat glands. Deficiencies in its signaling have been shown to be involved in patients affected by anhidrotic/hypohidrotic ectodermal dysplasia (2). This antibody is recommended for applications under non-denaturing conditions.
References
1. Kowalczyk-Quintas C and Schneider P. (2014). Ectodysplasin A (EDA) - EDA receptor signalling and its pharmacological modulation. Cytokine Growth Factor Rev;25(2):195-203. doi: 10.1016/j.cytogfr.2014.01.004. Epub 2014 Jan 23
2. Sadier A., Viriot L., Pantalacci S and Laudet V. (2014). The ectodysplasin pathway: from diseases to adaptations. Trends Genet Jan;30(1):24-31. doi: 10.1016/j.tig.2013.08.006. Epub 2013 Sep 23.
2. Sadier A., Viriot L., Pantalacci S and Laudet V. (2014). The ectodysplasin pathway: from diseases to adaptations. Trends Genet Jan;30(1):24-31. doi: 10.1016/j.tig.2013.08.006. Epub 2013 Sep 23.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
51,690 Da
NCBI Official Full Name
Ectodysplasin A receptor
NCBI Official Synonym Full Names
ectodysplasin A receptor
NCBI Official Symbol
EDAR
NCBI Official Synonym Symbols
DL; ED3; ED5; ED1R; EDA3; HRM1; EDA1R; ECTD10A; ECTD10B; EDA-A1R
NCBI Protein Information
tumor necrosis factor receptor superfamily member EDAR
UniProt Protein Name
Tumor necrosis factor receptor superfamily member EDAR
UniProt Gene Name
EDAR
UniProt Synonym Gene Names
DL
UniProt Entry Name
EDAR_HUMAN
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Product Notes
The EDAR edar (Catalog #AAA71496) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Ectodysplasin A Receptor (EDAR) can be used in a range of immunoassay formats including, but not limited to, ELISA, ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the EDAR edar for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ectodysplasin A Receptor (EDAR), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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