IF (Immunofluorescence)
(Immunofluorescent analysis of EG5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)
Rabbit EG5 Monoclonal Antibody | anti-EG5 antibody
Anti-EG5 Antibody
Gene Names
KIF11; EG5; HKSP; KNSL1; MCLMR; TRIP5
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
EG5, Antibody; Anti-EG5 Antibody; anti-EG5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Specificity
Recognizes endogenous levels of EG5 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 50mM Tris-Glycine (pH7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.
Applicable Applications for anti-EG5 antibody
IC (Immunochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
Recombinant protein of human Eg5
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescent analysis of EG5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)
IF (Immunofluorescence)
(Immunofluorescent analysis of EG5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)
WB (Western Blot)
(Western blot analysis of EG5 expression in NIH3T3 (A), MEF (B), Hela (C), A549 (D), HL60 (E), U2OS (F), C6 (G) whole cell lysates. (Predicted band size: 119 kD; Observed band size: 119 kD))
WB (Western Blot)
(Western blot analysis of EG5 expression in NIH3T3 (A), MEF (B), Hela (C), A549 (D), HL60 (E), U2OS (F), C6 (G) whole cell lysates. (Predicted band size: 119 kD; Observed band size: 119 kD))
Related Product Information for anti-EG5 antibody
Rabbit monoclonal antibody to EG5
Product Categories/Family for anti-EG5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
119,159 Da
NCBI Official Full Name
kinesin-like protein KIF11
NCBI Official Synonym Full Names
kinesin family member 11
NCBI Official Symbol
KIF11
NCBI Official Synonym Symbols
EG5; HKSP; KNSL1; MCLMR; TRIP5
NCBI Protein Information
kinesin-like protein KIF11; TRIP-5; kinesin-like protein 1; TR-interacting protein 5; kinesin-like spindle protein HKSP; kinesin-related motor protein Eg5; thyroid receptor-interacting protein 5
UniProt Protein Name
Kinesin-like protein KIF11
UniProt Gene Name
KIF11
UniProt Synonym Gene Names
EG5; KNSL1; TRIP5; TR-interacting protein 5; TRIP-5
UniProt Entry Name
KIF11_HUMAN
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Product Notes
The EG5 kif11 (Catalog #AAA265716) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-EG5 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EG5 can be used in a range of immunoassay formats including, but not limited to, IC (Immunochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the EG5 kif11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EG5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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