<
IHC (Immunohistochemistry)
(At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Rabbit Eg5 Polyclonal Antibody | anti-Eg5 antibody
Phospho-Eg5 (Thr926) Antibody
Gene Names
KIF11; EG5; HKSP; KNSL1; MCLMR; TRIP5
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig (83%), Zebrafish (83%), Bovine (92%), Horse (92%), Sheep (92%), Rabbit (92%), Dog (83%), Chicken (83%)
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
Eg5, Antibody; Phospho-Eg5 (Thr926) Antibody; EG5; HKSP; KIF11; KIF11_HUMAN; Kinesin family member 11; Kinesin like protein 1; Kinesin-like protein 1; Kinesin-like protein KIF11; Kinesin-like spindle protein HKSP; Kinesin-related motor protein Eg5; KNSL1; MCLMR; Thyroid receptor-interacting protein 5; TR-interacting protein 5; TRIP-5; TRIP5; anti-Eg5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (83%), Zebrafish (83%), Bovine (92%), Horse (92%), Sheep (92%), Rabbit (92%), Dog (83%), Chicken (83%)
Predicted Reactivity: Pig (83%), Zebrafish (83%), Bovine (92%), Horse (92%), Sheep (92%), Rabbit (92%), Dog (83%), Chicken (83%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-Eg5 (Thr926) Antibody detects endogenous levels of Eg5 only when phosphorylated at Thr926.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-Eg5 antibody
ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human Eg5 around the phosphorylation site of Thr926.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Phosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase). A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function.
Subunit Structure
Interacts with the thyroid hormone receptor in the presence of thyroid hormone. Component of a large chromatin remodeling complex, at least composed of MYSM1, PCAF, RBM10 and KIF11/TRIP5. Interacts (via C-terminus) with the kinase NEK6 in both interphase and mitosis.
Similarity
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. BimC subfamily.
Subcellular Location
Cytoplasm. Cytoplasm>Cytoskeleton>Spindle pole.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohistochemistry)
(At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemistry)
(At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(At 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(At 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from COLO205, using Phospho-KIF11/Eg5 (Thr926) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from COLO205, using Phospho-KIF11/Eg5 (Thr926) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using Phospho-KIF11/Eg5 (Thr926) Antibody.Lane 1: A2780 cells straved, blocked with antigen-specific peptides,Lane 2: A2780 cells straved,Lane 3: LPS treated RAW264.7 cells.)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using Phospho-KIF11/Eg5 (Thr926) Antibody.Lane 1: A2780 cells straved, blocked with antigen-specific peptides,Lane 2: A2780 cells straved,Lane 3: LPS treated RAW264.7 cells.)
Related Product Information for anti-Eg5 antibody
Motor protein required for establishing a bipolar spindle during mitosis. Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
119,159 Da
NCBI Official Full Name
kinesin-like protein KIF11
NCBI Official Synonym Full Names
kinesin family member 11
NCBI Official Symbol
KIF11
NCBI Official Synonym Symbols
EG5; HKSP; KNSL1; MCLMR; TRIP5
NCBI Protein Information
kinesin-like protein KIF11; TRIP-5; kinesin-like protein 1; TR-interacting protein 5; kinesin-like spindle protein HKSP; kinesin-related motor protein Eg5; thyroid receptor-interacting protein 5
UniProt Protein Name
Kinesin-like protein KIF11
UniProt Gene Name
KIF11
UniProt Synonym Gene Names
EG5; KNSL1; TRIP5; TR-interacting protein 5; TRIP-5
UniProt Entry Name
KIF11_HUMAN
Similar Products
Product Notes
The Eg5 kif11 (Catalog #AAA329462) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-Eg5 (Thr926) Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig (83%), Zebrafish (83%), Bovine (92%), Horse (92%), Sheep (92%), Rabbit (92%), Dog (83%), Chicken (83%) and may cross-react with other species as described in the data sheet. AAA Biotech's Eg5 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the Eg5 kif11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Eg5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.