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WB (Western Blot)
(EGFR Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the EGFR antibody detected the EGFR protein (arrow).)
Mouse anti-Human EGFR Monoclonal Antibody | anti-EGFR antibody
EGFR Antibody (C-term)
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry
Synonyms
EGFR; Monoclonal Antibody; EGFR Antibody (C-term); Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; ERBB; ERBB1; HER1; anti-EGFR antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
688CT33.1.3
Specificity
This EGFR antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 1163-1191 amino acids from the C-terminal region of human EGFR.
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
1163-1191
Sequence Length
1210
Applicable Applications for anti-EGFR antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
IHC~~1:25
WB~~1:1000
WB~~1:1000
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(EGFR Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the EGFR antibody detected the EGFR protein (arrow).)
WB (Western Blot)
(EGFR Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the EGFR antibody detected the EGFR protein (arrow).)
WB (Western Blot)
(Western blot analysis of lysates from A431, MCF-7 cell line (from left to right), using EGFR Antibody (C-term). AAA28637 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from A431, MCF-7 cell line (from left to right), using EGFR Antibody (C-term). AAA28637 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded R. esophagus section using EGFR Antibody (C-term). AAA28637 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded R. esophagus section using EGFR Antibody (C-term). AAA28637 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded M. esophagus section using EGFR Antibody (C-term). AAA28637 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded M. esophagus section using EGFR Antibody (C-term). AAA28637 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. stomach section using EGFR Antibody (C-term). AAA28637 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. stomach section using EGFR Antibody (C-term). AAA28637 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. esophagus section using EGFR Antibody (C-term). AAA28637 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. esophagus section using EGFR Antibody (C-term). AAA28637 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Related Product Information for anti-EGFR antibody
The protein encoded by this gene is a transmembrane
glycoprotein that is a member of the protein kinase superfamily.
This protein is a receptor for members of the epidermal growth
factor family. EGFR is a cell surface protein that binds to
epidermal growth factor. Binding of the protein to a ligand induces
receptor dimerization and tyrosine autophosphorylation and leads to
cell proliferation. Mutations in this gene are associated with lung
cancer. Multiple alternatively spliced transcript variants that
encode different protein isoforms have been found for this gene.
glycoprotein that is a member of the protein kinase superfamily.
This protein is a receptor for members of the epidermal growth
factor family. EGFR is a cell surface protein that binds to
epidermal growth factor. Binding of the protein to a ligand induces
receptor dimerization and tyrosine autophosphorylation and leads to
cell proliferation. Mutations in this gene are associated with lung
cancer. Multiple alternatively spliced transcript variants that
encode different protein isoforms have been found for this gene.
Product Categories/Family for anti-EGFR antibody
References
Abdallah, R.T., et al. J. Biol. Chem. 285(45):35206-35215(2010)
Lu, C., et al. Mol. Cell. Biol. 30(22):5432-5443(2010)
Rosell, R., et al. Ann. N. Y. Acad. Sci. 1210, 45-52 (2010) :
Hata, A., et al. J Thorac Oncol 5(10):1524-1528(2010)
Aguirre, A., et al. Nature 467(7313):323-327(2010)
Lu, C., et al. Mol. Cell. Biol. 30(22):5432-5443(2010)
Rosell, R., et al. Ann. N. Y. Acad. Sci. 1210, 45-52 (2010) :
Hata, A., et al. J Thorac Oncol 5(10):1524-1528(2010)
Aguirre, A., et al. Nature 467(7313):323-327(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
134277
NCBI Official Full Name
epidermal growth factor receptor isoform a
NCBI Official Synonym Full Names
epidermal growth factor receptor
NCBI Official Symbol
EGFR
NCBI Official Synonym Symbols
ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
NCBI Protein Information
epidermal growth factor receptor
UniProt Protein Name
Epidermal growth factor receptor
UniProt Gene Name
EGFR
UniProt Synonym Gene Names
ERBB; ERBB1; HER1
UniProt Entry Name
EGFR_HUMAN
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Product Notes
The EGFR egfr (Catalog #AAA28637) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1163-1191. The EGFR Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EGFR can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). IHC~~1:25 WB~~1:1000. Researchers should empirically determine the suitability of the EGFR egfr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EGFR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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