WB (Western Blot)
(All lanes : Anti-EIF2AK3 Antibody at 1:250-1:1000 dilutionLane 1: A549 whole cell lysateLane 2: PC-12 whole cell lysateLane 3: mouse brain lysateLane 4: mouse liver lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 125 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Mouse EIF2AK3 Monoclonal Antibody | anti-EIF2AK3 antibody
EIF2AK3 Antibody
Gene Names
EIF2AK3; PEK; WRS; PERK
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot
Synonyms
EIF2AK3, Antibody; EIF2AK3 Antibody; Eukaryotic translation initiation factor 2-alpha kinase 3; 2.7.11.1; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK; EIF2AK3; PEK; PERK; anti-EIF2AK3 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1385CT582.4.39
Specificity
This EIF2AK3 antibody is generated from a mouse immunized with a recombinant protein conjugated synthetic peptide between 530-850 amino acids from human EIF2AK3.
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Sequence Length
1116
Applicable Applications for anti-EIF2AK3 antibody
ELISA, WB (Western Blot)
Cellular Location
Endoplasmic reticulum membrane; Single-pass type I membrane protein.
Function
Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2Sl) on 'Ser-52' during the unfolded protein response (UPR) and in response to low amino acid availability. Converts phosphorylated eIF-2-alpha/EIF2Sl either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Serves as a critical effector of unfolded protein response (UPR)-induced Gl growth arrest due to the loss of cyclin-Dl (CCNDl). Involved in control of mitochondrial morphology and function.
Tissue Location
Ubiquitous. A high level expression is seen in secretory tissues
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(All lanes : Anti-EIF2AK3 Antibody at 1:250-1:1000 dilutionLane 1: A549 whole cell lysateLane 2: PC-12 whole cell lysateLane 3: mouse brain lysateLane 4: mouse liver lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 125 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-EIF2AK3 Antibody at 1:250-1:1000 dilutionLane 1: A549 whole cell lysateLane 2: PC-12 whole cell lysateLane 3: mouse brain lysateLane 4: mouse liver lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 125 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-EIF2AK3 antibody
Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1(CCND1). Involved in control of mitochondrial morphology and function (By similarity).
Product Categories/Family for anti-EIF2AK3 antibody
References
Shi Y.,et al.J. Biol. Chem. 274:5723-5730 (1999). Sood R.,et al.Biochem. J. 346:281-293 (2000). Delepine M.,et al.Nat. Genet. 25:406-409 (2000). Ota T.,et al.Nat. Genet. 36:40-45 (2004). Hillier L.W.,et al.Nature 434:724-731 (2005).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
125216 Da
NCBI Official Full Name
eukaryotic translation initiation factor 2-alpha kinase 3 isoform 1
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 2 alpha kinase 3
NCBI Official Symbol
EIF2AK3
NCBI Official Synonym Symbols
PEK; WRS; PERK
NCBI Protein Information
eukaryotic translation initiation factor 2-alpha kinase 3
UniProt Protein Name
Eukaryotic translation initiation factor 2-alpha kinase 3
UniProt Gene Name
EIF2AK3
UniProt Synonym Gene Names
PEK; PERK; HsPEK
UniProt Entry Name
E2AK3_HUMAN
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Product Notes
The EIF2AK3 eif2ak3 (Catalog #AAA290655) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EIF2AK3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EIF2AK3 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the EIF2AK3 eif2ak3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF2AK3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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