IF (Immunofluorescence)
(Immunofluorescent analysis of Hela cells fixed by anhydrous methanol for 2 h at -20? and using anti-eIF2? mouse mAb (dilution 1:200).)
Mouse eIF2alpha Monoclonal Antibody | anti-eIF2alpha antibody
eIF2alpha Mouse mAb
Gene Names
EIF2S1; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
Reactivity
Human, Mouse, Rat
Applications
Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Synonyms
eIF2alpha, Antibody; eIF2alpha Mouse mAb; Dmel/CG6376; Dmel_CG6376; drosE2F1; E(Sev-CycE)3A; E(var)3-93E; E2-promoter binding facto; E2F 1; E2F transcription factor 1; E2F-1; E2f-PA; E2f-PB; E2f-PC; E2F1; E2f1 E2F transcription factor 1; E2F1_HUMAN; Evar(3)164; KIAA4009; l(3)07172; l(3)j3B1; l(3)j3C2; l(3)rM729; mKIAA4009; OTTHUMP00000030661; PBR3; PRB binding protein E2F 1; PRB-binding protein E2F-1; RBAP 1; RBAP-1; RBAP1; RBBP-3; RBBP3; RBP 3; RBP3; Retinoblastoma-associated protein 1; Retinoblastoma-binding protein 3; Transcription factor E2F1.; anti-eIF2alpha antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Specificity
This antibody detects endogenous levels of eIF2alpha and does not cross-react with related proteins.
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-eIF2alpha antibody
IP (Immunoprecipitation), ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
Purified recombinant human eIF2alpha protein fragments expressed in E coli.
Modification
Unmodified/Total
Preparation and Storage
Store at -20 degree C/1 year
IF (Immunofluorescence)
(Immunofluorescent analysis of Hela cells fixed by anhydrous methanol for 2 h at -20? and using anti-eIF2? mouse mAb (dilution 1:200).)
IF (Immunofluorescence)
(Immunofluorescent analysis of Hela cells fixed by anhydrous methanol for 2 h at -20? and using anti-eIF2? mouse mAb (dilution 1:200).)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of Hela cell lysates using eIF2?.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of Hela cell lysates using eIF2?.)
WB (Western Blot)
(Western blot detection of eIF2? in K562.COS7.3T3. C2C12.MCF-7.C6 and Hela cell lysates using eIF2? mouse mAb (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.)
WB (Western Blot)
(Western blot detection of eIF2? in K562.COS7.3T3. C2C12.MCF-7.C6 and Hela cell lysates using eIF2? mouse mAb (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.)
Related Product Information for anti-eIF2alpha antibody
The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]
Product Categories/Family for anti-eIF2alpha antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38 kDa
NCBI Official Full Name
eukaryotic translation initiation factor 2 subunit 1
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
NCBI Official Symbol
EIF2S1
NCBI Official Synonym Symbols
EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
NCBI Protein Information
eukaryotic translation initiation factor 2 subunit 1; eIF-2-alpha; eukaryotic translation initiation factor 2 subunit alpha
UniProt Protein Name
Eukaryotic translation initiation factor 2 subunit 1
UniProt Gene Name
EIF2S1
UniProt Synonym Gene Names
EIF2A; eIF-2-alpha; eIF-2A; eIF-2alpha
UniProt Entry Name
IF2A_HUMAN
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Product Notes
The eIF2alpha eif2s1 (Catalog #AAA320398) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The eIF2alpha Mouse mAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's eIF2alpha can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the eIF2alpha eif2s1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "eIF2alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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