IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of HeLa cells treated by CA using 2.5ug Phospho-eIF2alpha-S51 antibody. Western blot was performed from the immunoprecipitate using Phospho-eIF2alpha-S51 antibody at a dilition of 1:1000.)
Rabbit anti-Human eIF2alpha-S51 Polyclonal Antibody | anti-eIF2alpha-S51 antibody
Phospho-eIF2alpha-S51 pAb
Gene Names
EIF2S1; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Affinity Purification
Synonyms
eIF2alpha-S51, Antibody; Phospho-eIF2alpha-S51 pAb; EIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A; anti-eIF2alpha-S51 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Sequence
SELSR
Sequence Length
315
Applicable Applications for anti-eIF2alpha-S51 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
A phospho specific peptide corresponding to residues surrounding S51 of human eIF2alpha
Immunogen Species
Human
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular Location
Cytoplasmic granule
Positive Samples
HeLa, Jurkat
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of HeLa cells treated by CA using 2.5ug Phospho-eIF2alpha-S51 antibody. Western blot was performed from the immunoprecipitate using Phospho-eIF2alpha-S51 antibody at a dilition of 1:1000.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of HeLa cells treated by CA using 2.5ug Phospho-eIF2alpha-S51 antibody. Western blot was performed from the immunoprecipitate using Phospho-eIF2alpha-S51 antibody at a dilition of 1:1000.)
WB (Western Blot)
(Western blot analysis of extracts of HeLa and Jurkat cells, using Phospho-eIF2alpha-S51 antibody at 1:2000 dilution. HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight. Jurkat cells were treated by Calyculin A (100nM) for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 1s.)
WB (Western Blot)
(Western blot analysis of extracts of HeLa and Jurkat cells, using Phospho-eIF2alpha-S51 antibody at 1:2000 dilution. HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight. Jurkat cells were treated by Calyculin A (100nM) for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 1s.)
Product Categories/Family for anti-eIF2alpha-S51 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Calculated: 36kDa
Observed: 38kDa
Observed: 38kDa
NCBI Official Full Name
eukaryotic translation initiation factor 2 subunit 1
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 2 subunit alpha
NCBI Official Symbol
EIF2S1
NCBI Official Synonym Symbols
EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
NCBI Protein Information
eukaryotic translation initiation factor 2 subunit 1
UniProt Protein Name
Eukaryotic translation initiation factor 2 subunit 1
UniProt Gene Name
EIF2S1
UniProt Synonym Gene Names
EIF2A; eIF-2-alpha; eIF-2A; eIF-2alpha
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Product Notes
The eIF2alpha-S51 eif2s1 (Catalog #AAA281280) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-eIF2alpha-S51 pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's eIF2alpha-S51 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the eIF2alpha-S51 eif2s1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SELSR. It is sometimes possible for the material contained within the vial of "eIF2alpha-S51, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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