WB (Western Blot)
(EIF4E Antibody western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the EIF4E antibody detected the EIF4E protein (arrow).)
Mouse anti-Human EIF4E Monoclonal Antibody | anti-EIF4E antibody
EIF4E Antibody
Gene Names
EIF4E; CBP; EIF4F; AUTS19; EIF4E1; EIF4EL1
Reactivity
Human
Applications
Immunofluorescence, ELISA, Western Blot
Synonyms
EIF4E, Antibody; EIF4E Antibody; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein; EIF4E; EIF4EL1; EIF4F; anti-EIF4E antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,K
Clone Number
163CT48.1.9
Specificity
This EIF4E monoclonal antibody is generated from mouse immunized with EIF4E recombinant protein.
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Sequence Length
237
Applicable Applications for anti-EIF4E antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Recombinant Protein
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(EIF4E Antibody western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the EIF4E antibody detected the EIF4E protein (arrow).)
WB (Western Blot)
(EIF4E Antibody western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the EIF4E antibody detected the EIF4E protein (arrow).)
IF (Immunofluorescence)
(Fluorescent image of Hela cell stained with EIF4E Antibody. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EIF4E primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). EIF4E immunoreactivity is localized to Cytoplasm significantly.)
IF (Immunofluorescence)
(Fluorescent image of Hela cell stained with EIF4E Antibody. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EIF4E primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). EIF4E immunoreactivity is localized to Cytoplasm significantly.)
Related Product Information for anti-EIF4E antibody
EIF4E recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25097
NCBI Official Full Name
eukaryotic translation initiation factor 4E isoform 3
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4E
NCBI Official Symbol
EIF4E
NCBI Official Synonym Symbols
CBP; EIF4F; AUTS19; EIF4E1; EIF4EL1
NCBI Protein Information
eukaryotic translation initiation factor 4E
UniProt Protein Name
Eukaryotic translation initiation factor 4E
UniProt Gene Name
EIF4E
UniProt Synonym Gene Names
EIF4EL1; EIF4F; eIF-4E; eIF4E
UniProt Entry Name
IF4E_HUMAN
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Product Notes
The EIF4E eif4e (Catalog #AAA283597) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EIF4E Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EIF4E can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the EIF4E eif4e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF4E, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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