Mouse Endoglin/CD105 Monoclonal Antibody | anti-ENG antibody

Endoglin/CD105 (Angiogenesis Marker)

Cat. Num. AAA215474

• Gene Names: ENG; END; HHT1; ORW1
• Reactivity: Human, Monkey, Horse, Pig, Rat
• Applications: Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
• Purity: Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA at 1.0mg/ml
• Synonyms: Endoglin/CD105, Antibody; Endoglin/CD105 (Angiogenesis Marker); CD105; END; Endoglin; Eng; HHT1; S-endoglin; anti-ENG antibody

• Host: Mouse
• Reactivity: Human, Monkey, Horse, Pig, Rat
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: ENG/1621
• Specificity: CD105/Endoglin is a Type I membrane glycoprotein located on cell surfaces and is part of the TGF-beta receptor complex. This protein has been found on endothelial cells, activated macrophages, fibroblasts, and smooth-muscle cells. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. CD105 is highly expressed in endothelial cells during tumor angiogenesis and inflammation, with weak or negative expression in vascular endothelium of normal tissues. Angiogenesis is a promising prognostic marker in a variety of tumors. Endoglin is a more specific and sensitive marker for tumor angiogenesis than CD31 or CD34, as it labels only newly-formed blood vessels and may serve as a prognostic marker for Prostate Adenocarcinoma, and cancers of the lung, stomach, breast, and brain.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA at 1.0mg/ml
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 625

• Applicable Applications for anti-ENG antibody: IF (Immunofluorescence), FCM/FACS (Flow Cytometry), ELISA
• Immunogen: Recombinant extracellular fragment of Human Endoglin protein exact sequence is proprietary)
• Cellular Localization: Cell Surface
• Hu-chromosome Location: 9q34.11
• Positive Control: HeLa, A431 or HL-60 cells.
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis Purified Endoglin / CD105 Mouse Monoclonal Antibody (ENG/1621).Confirmation of Integrity and Purity of Antibody.)

FCM/FACS (Flow Cytometry)

(Flow Cytometric Analysis of trypsinized PFA-fixed HeLa cells. Endoglin / CD105 Mouse Monoclonal Antibody (ENG/1621) followed by goat anti-mouse IgG-CF488 (Blue); Isotype control (Red).)

IF (Immunofluorescence)

(Immunofluorescent staining of PFA-fixed HeLa cells with Endoglin / CD105 Mouse Monoclonal Antibody (ENG/1621) followed by goat anti-mouse IgG-CF488 (Green).)

References

Altomonte, M., et al. 1996. Expression and structural features of Endoglin (CD105), a transforming growth factor 3 binding protein, in human melanoma. Br. J. Cancer 74: 1586-1591.

NCBI and Uniprot Product Information

• NCBI GI #: 4557555
• NCBI GeneID: 2022
• NCBI Accession #: NP_000109.1
• NCBI GenBank Nucleotide #: NP_000109.1
• UniProt Accession #: P17813
• Molecular Weight: 80kDa (Monomer); 160kDa (Homodimer)
• NCBI Official Full Name: endoglin isoform 2
• NCBI Official Synonym Full Names: endoglin
• NCBI Official Symbol: ENG
• NCBI Official Synonym Symbols: END; HHT1; ORW1
• NCBI Protein Information: endoglin
• UniProt Protein Name: Endoglin
• UniProt Gene Name: ENG
• UniProt Synonym Gene Names: END
• UniProt Entry Name: EGLN_HUMAN