Mouse ENO1 Monoclonal Antibody | anti-ENO1 antibody

ENO1 Monoclonal Antibody

Cat. Num. AAA27044

• Gene Names: ENO1; NNE; PPH; MPB1; ENO1L1
• Reactivity: Human, Mouse, Rat, Rabbit
• Applications: ELISA, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay, Immunoprecipitation
• Purity: >95%, Protein G purified
• Synonyms: ENO1, Antibody; ENO1 Monoclonal Antibody; Alpha-enolase (2-phospho-D-glycerate hydro-lyase) (C-myc promoter-binding protein) (Enolase 1) (MBP-1) (MPB-1) (Non-neural enolase) (NNE) (Phosphopyruvate hydratase) (Plasminogen-binding protein); ENO1; ENO1L1; MBPB1; MPB1; anti-ENO1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Rabbit
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 4D11F5
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid; Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH7.4

• Applicable Applications for anti-ENO1 antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
• Application Notes: WB: 1:5000-1:640,000; IF: 1:50-1:300; FC/FACS: 1:100-1:600; IP: 1ul-4ul
• Conjugation: Non-conjugated
• Immunogen: Recombinant Human Alpha-enolase protein (2-434AA)
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

IP (Immunoprecipitation)

(Immunoprecipitating ENO1 in HepG2 whole cell lysate Lane 1: Mouse control IgG (1ug) instead of in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/5000) Lane 2: (1ul) + HepG2 whole cell lysate (500ug) Lane 3: HepG2 whole cell lysate (10ug))

FCM (Flow Cytometry)

(Overlay histogram showing Hela cells stained with (red line) at 1:550. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)

FCM (Flow Cytometry)

(Overlay histogram showing MCF-7 cells stained with (red line) at 1:550. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)

IF (Immunofluorescence)

(Immunofluorescence staining of Hela cells at 1:270, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).)

IF (Immunofluorescence)

(Immunofluorescence staining of MCF-7 cells at 1:270, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).)

WB (Western Blot)

(Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: ENO1 antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 47 kDa Observed band size: 47 KDa Exposure time: 10s)

WB (Western Blot)

(Western Blot Positive WB detected in: HepG2 whole cell lysate at 20ug, 10ug, 5ug, 2.5ug, 1.25ug, 0.625ug All lanes: ENO1 antibody at 1:5000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 47 kDa Observed band size: 47 KDa Exposure time: 10s)

WB (Western Blot)

(Western Blot Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate All lanes ENO1 antibody at 1:10000 Secondary Goat polyclonal to mouse IgG at 0.261ug/ml Predicted band size: 47 KDa Observed band size: 47 KDa Exposure time: 10s)

WB (Western Blot)

(Western Blot Positive WB detected in: K562 whole cell lysate, Rabbit Skeletal Muscle tissue, Rabbit Kidney lysate All lanes ENO1 antibody at 1:10000 Secondary Goat polyclonal to mouse IgG at 0.261ug/ml Predicted band size: 47 KDa Observed band size: 47 KDa Exposure time: 1min)

Related Product Information for anti-ENO1 antibody

ENO1 encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.

Product Categories/Family for anti-ENO1 antibody

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 319996655
• NCBI GeneID: 2023
• NCBI Accession #: NP_001188412.1
• NCBI GenBank Nucleotide #: NM_001201483.1
• UniProt Accession #: P06733; P22712; Q16704; Q4TUS4; Q53FT9; Q53HR3; Q658M5; Q6GMP2; Q71V37; Q7Z3V6; Q8WU71; B2RD59
• Molecular Weight: 434 (341)
• NCBI Official Full Name: c-myc promoter-binding protein-1 isoform MBP-1
• NCBI Official Synonym Full Names: enolase 1, (alpha)
• NCBI Official Symbol: ENO1
• NCBI Official Synonym Symbols: NNE; PPH; MPB1; ENO1L1
• NCBI Protein Information: alpha-enolase; c-myc promoter-binding protein-1; enolase-alpha; tau-crystallin; non-neural enolase; alpha enolase like 1; phosphopyruvate hydratase; plasminogen-binding protein; MYC promoter-binding protein 1; 2-phospho-D-glycerate hydro-lyase
• UniProt Protein Name: Alpha-enolase
• UniProt Gene Name: ENO1
• UniProt Synonym Gene Names: ENO1L1; MBPB1; MPB1; NNE
• UniProt Entry Name: ENOA_HUMAN