WB (Western Blot)
(Western blot analysis of lysates from 1) Rat Heart Tissue. 2) huvec.3) Jurkat cells. (Green) primary antibody was diluted at 1:1000. 4 degree over night. secondary antibodywas diluted at 1:10000. 37 degree 1hour. (Red) Actin ? Polyclonal Antibody antibody was diluted at 1:5000 as loading control. 4 degree over night.secondary antibodywas diluted at 1:10000. 37 degree 1hour.)
Mouse eNOS Monoclonal Antibody | anti-eNOS antibody
eNOS Monoclonal Antibody (Mix)
Gene Names
NOS3; eNOS; ECNOS
Reactivity
Human, Mouse, Rat, Rabbit
Applications
Western Blot
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Synonyms
eNOS, Antibody; eNOS Monoclonal Antibody (Mix); Nitric oxide synthase, endothelial; NOS3; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII; anti-eNOS antibody
Host
Mouse
Reactivity
Human, Mouse, Rat, Rabbit
Clonality
Monoclonal
Clone Number
Mix
Specificity
The antibody detects endogenous eNOS protein.
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Form/Format
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Applicable Applications for anti-eNOS antibody
WB (Western Blot)
Immunogen
Recombinant Protein of eNOS
Modification
Unmodified/Total
Preparation and Storage
Store at -20 degree C/1 year
WB (Western Blot)
(Western blot analysis of lysates from 1) Rat Heart Tissue. 2) huvec.3) Jurkat cells. (Green) primary antibody was diluted at 1:1000. 4 degree over night. secondary antibodywas diluted at 1:10000. 37 degree 1hour. (Red) Actin ? Polyclonal Antibody antibody was diluted at 1:5000 as loading control. 4 degree over night.secondary antibodywas diluted at 1:10000. 37 degree 1hour.)
WB (Western Blot)
(Western blot analysis of lysates from 1) Rat Heart Tissue. 2) huvec.3) Jurkat cells. (Green) primary antibody was diluted at 1:1000. 4 degree over night. secondary antibodywas diluted at 1:10000. 37 degree 1hour. (Red) Actin ? Polyclonal Antibody antibody was diluted at 1:5000 as loading control. 4 degree over night.secondary antibodywas diluted at 1:10000. 37 degree 1hour.)
Application Data
(The picture was kindly provided by our customer)
Application Data
(The picture was kindly provided by our customer)
Related Product Information for anti-eNOS antibody
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
Product Categories/Family for anti-eNOS antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
133,289 Da
NCBI Official Full Name
nitric oxide synthase, endothelial isoform 1
NCBI Official Synonym Full Names
nitric oxide synthase 3 (endothelial cell)
NCBI Official Symbol
NOS3
NCBI Official Synonym Symbols
eNOS; ECNOS
NCBI Protein Information
nitric oxide synthase, endothelial; cNOS; EC-NOS; NOSIII; NOS type III; endothelial NOS; constitutive NOS
UniProt Protein Name
Nitric oxide synthase, endothelial
UniProt Gene Name
NOS3
UniProt Synonym Gene Names
cNOS; eNOS; NOSIII
UniProt Entry Name
NOS3_HUMAN
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Product Notes
The eNOS nos3 (Catalog #AAA320452) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The eNOS Monoclonal Antibody (Mix) reacts with Human, Mouse, Rat, Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's eNOS can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the eNOS nos3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "eNOS, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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