Mouse anti-Human ER-beta1 (Estrogen Receptor beta-1) Monoclonal Antibody | anti-ESR2 antibody

ER-beta1 (Estrogen Receptor beta-1) Mouse Monoclonal Antibody

Cat. Num. AAA13846

• Gene Names: ESR2; Erb; ESRB; ESTRB; NR3A2; ER-BETA; ESR-BETA
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
• Synonyms: ER-beta1 (Estrogen Receptor beta-1), Antibody; ER-beta1 (Estrogen Receptor beta-1) Mouse Monoclonal Antibody; Erb, ESR BETA, ESR2, ESRB, ESTRB, estrogen nuclear receptor beta variant a, estrogen nuclear receptor beta variant b, estrogen receptor 2 (ER beta), estrogen receptor beta 4; anti-ESR2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: ESR2/686
• Specificity: Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER-alpha and ER-beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER-alpha and ER-beta are differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER-alpha and ER-beta may be regulated by distinct mechanisms even though they share many functional characteristics.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 495

• Applicable Applications for anti-ESR2 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (0.5-1ug/ml); Western Blot (0.5-1ug/ml); Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Predominantly nuclear
• Immunogen: C-terminus fragment of recombinant human estrogen receptor beta protein
• Hu-Chromosome Location: 14q23.2
• Positive Control: MCF-7 Cells. Ovarian, Breast, Bladder, Gastric or Salivary carcinoma
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(Purified ER-beta Mouse Monoclonal Antibody (ESR2/686). Confirmation of Integrity and Purity of Antibody.)

FCM (Flow Cytometry)

(Flow Cytometry for human ER-beta on MCF-7 Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled ER-beta1 Monoclonal Antibody (ESR2/686).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with ER-beta1 Monoclonal Antibody (ESR2/686).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with ER-beta1 Monoclonal Antibody (ESR2/686).)

FCM (Flow Cytometry)

(Flow Cytometry of human ER beta on BT474 Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled ER beta1 Monoclonal Antibody (ESR2/686).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with ER-beta1 Monoclonal Antibody (ESR2/686).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with ER-beta1 Monoclonal Antibody (ESR2/686).)

References

Skliris GP et. al. J Pathol 2002;197:155-62.

NCBI and Uniprot Product Information

• NCBI GI #: 94538325
• NCBI GeneID: 2100
• NCBI Accession #: NP_001035365.1
• NCBI GenBank Nucleotide #: NM_001040275.1
• UniProt Accession #: Q92731; O60608; O60685; O60702; O60703; O75583; O75584; Q0MWT5; Q0MWT6; Q86Z31; A8K8K5; G3V5M5
• Molecular Weight: 53-59 kDa
• NCBI Official Full Name: estrogen receptor beta isoform 2
• NCBI Official Synonym Full Names: estrogen receptor 2
• NCBI Official Symbol: ESR2
• NCBI Official Synonym Symbols: Erb; ESRB; ESTRB; NR3A2; ER-BETA; ESR-BETA
• NCBI Protein Information: estrogen receptor beta
• UniProt Protein Name: Estrogen receptor beta
• UniProt Gene Name: ESR2
• UniProt Synonym Gene Names: ESTRB; NR3A2; ER-beta
• UniProt Entry Name: ESR2_HUMAN