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Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using ERCC1 Mouse Monoclonal Antibody (ERCC1/2318). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Mouse anti-Human ERCC1/RAD10 Monoclonal Antibody | anti-ERCC1 antibody
ERCC1/RAD10 (Tumor Progression Marker)
Gene Names
ERCC1; UV20; COFS4; RAD10
Reactivity
Human
Applications
Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
ERCC1/RAD10, Antibody; ERCC1/RAD10 (Tumor Progression Marker); COFS4; DNA excision repair protein ERCC1; ERCC1; Excision repair cross complementing 1; RAD10; UV20; anti-ERCC1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
ERCC1/2318
Specificity
Recognizes a protein of 110kDa, identified as Excision Repair Cross Complementing 1 (ERCC1). It is a mammalian nucleotide excision repair (NER) enzyme involved in repair of damaged DNA. ERCC1 is a homologous to RAD10 in Saccharomyces cerevisiae, which is required in mitotic intrachromosomal recombination and repair. ERCC1 is required in repair of cisplatin-induced DNA adducts and ultraviolet (UV)-induced DNA damage. High expression of ERCC1 has been linked to tumor progression in a variety of cancers including non-small cell lung cancer (NSCLC), squamous cell carcinoma of the head, ovarian cancer and esophageal cancer.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
273
Applicable Applications for anti-ERCC1 antibody
Immunohistochemistry (IHC) Paraffin
Application Notes
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Positive Control
HeLa, HepG2 cells. Tonsil or Prostate.
Immunogen
Recombinant fragment (around aa 191-281) of human ERCC1 protein (exact sequence is proprietary)
Cellular Localization
Nuclear
Hu-chromosome Location
19q13.32
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using ERCC1 Mouse Monoclonal Antibody (ERCC1/2318). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using ERCC1 Mouse Monoclonal Antibody (ERCC1/2318). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with ERCC1 Mouse Monoclonal Antibody (ERCC1/2318).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with ERCC1 Mouse Monoclonal Antibody (ERCC1/2318).)
SDS-PAGE
(SDS-PAGE Analysis Purified ERCC1 Mouse Monoclonal Antibody (ERCC1/2318). Confirmation of Purity and Integrity of Antibody.)
SDS-PAGE
(SDS-PAGE Analysis Purified ERCC1 Mouse Monoclonal Antibody (ERCC1/2318). Confirmation of Purity and Integrity of Antibody.)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with ERCC1 Mouse Monoclonal Antibody (ERCC1/2318).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with ERCC1 Mouse Monoclonal Antibody (ERCC1/2318).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Pancreas stained with ERCC1 Mouse Monoclonal Antibody (ERCC1/2318).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Pancreas stained with ERCC1 Mouse Monoclonal Antibody (ERCC1/2318).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Pancreas stained with ERCC1 Mouse Monoclonal Antibody (ERCC1/2318).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Pancreas stained with ERCC1 Mouse Monoclonal Antibody (ERCC1/2318).)
References
1. Hayashi T, et al. Mutat Res 1998;407:269-276. 2. Miura M, et al. Exp Cell Res 1996;226:126-132.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
110kDa
NCBI Official Full Name
DNA excision repair protein ERCC-1 isoform 3
NCBI Official Synonym Full Names
ERCC excision repair 1, endonuclease non-catalytic subunit
NCBI Official Symbol
ERCC1
NCBI Official Synonym Symbols
UV20; COFS4; RAD10
NCBI Protein Information
DNA excision repair protein ERCC-1
UniProt Protein Name
DNA excision repair protein ERCC-1
UniProt Gene Name
ERCC1
UniProt Entry Name
ERCC1_HUMAN
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Product Notes
The ERCC1 ercc1 (Catalog #AAA23913) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ERCC1/RAD10 (Tumor Progression Marker) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ERCC1/RAD10 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin. Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the ERCC1 ercc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERCC1/RAD10, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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