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WB (Western Blot)
(Western blot analysis of extracts from mouse brain, using ERCC1 Antibody.)
Rabbit anti-Human, Mouse ERCC1 Polyclonal Antibody | anti-ERCC1 antibody
ERCC1 Antibody
Gene Names
ERCC1; UV20; COFS4; RAD10
Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ERCC1, Antibody; ERCC1 Antibody; COFS 4; COFS4; DNA excision repair protein ERCC 1; DNA excision repair protein ERCC-1; DNA excision repair protein ERCC1; ERCC 1; ERCC1; ERCC1_HUMAN; Excision repair cross complementation group 1; Excision repair cross complementing 1; Excision Repair Cross Complementing Rodent Repair Deficiency Complementation Group 1; Excision repair protein; RAD 10; RAD10; UV 20; UV20; anti-ERCC1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
ERCC1 antibody detects endogenous levels of total ERCC1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
273
Applicable Applications for anti-ERCC1 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human ERCC1
Subcellular Location
Nucleus.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Xenopus
Similarity
Pig (100%), Zebrafish (80%), Bovine (100%), Horse (100%), Sheep (90%), Dog (100%), Xenopus (88%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from mouse brain, using ERCC1 Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from mouse brain, using ERCC1 Antibody.)
IF (Immunofluorescence)
(AAA320800 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IF (Immunofluorescence)
(AAA320800 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis on NIH-3T3 cell lysate using ERCC1 Antibody, The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis on NIH-3T3 cell lysate using ERCC1 Antibody, The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry)
(IHC analysis of mouse kidney tissue, using ERCC1 Antibody at 1:100.)
IHC (Immunohistochemistry)
(IHC analysis of mouse kidney tissue, using ERCC1 Antibody at 1:100.)
Related Product Information for anti-ERCC1 antibody
Description: ERCC1 a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA excision repair. Belongs to the ERCC1/RAD10/SWI10 family. Heterodimer composed of ERCC1 and XPF/ERRC4. Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4, a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord.
Function: Isoform 1: Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4.
Subunit Structure: Heterodimer composed of ERCC1 isoform 1 and XPF/ERRC4.
Similarity: Belongs to the ERCC1/RAD10/SWI10 family.
Function: Isoform 1: Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4.
Subunit Structure: Heterodimer composed of ERCC1 isoform 1 and XPF/ERRC4.
Similarity: Belongs to the ERCC1/RAD10/SWI10 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 36 kDa
Predicted: 33 kDa
Predicted: 33 kDa
NCBI Official Full Name
DNA excision repair protein ERCC-1 isoform 3
NCBI Official Synonym Full Names
ERCC excision repair 1, endonuclease non-catalytic subunit
NCBI Official Symbol
ERCC1
NCBI Official Synonym Symbols
UV20; COFS4; RAD10
NCBI Protein Information
DNA excision repair protein ERCC-1
UniProt Protein Name
DNA excision repair protein ERCC-1
UniProt Gene Name
ERCC1
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Product Notes
The ERCC1 ercc1 (Catalog #AAA320800) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ERCC1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ERCC1 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ERCC1 ercc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERCC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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