Rabbit ERCC1 Polyclonal Antibody | anti-ERCC1 antibody

Anti-ERCC1 Antibody Picoband

Cat. Num. AAA126016

• Gene Names: ERCC1; UV20; COFS4; RAD10
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Immunocytochemistry, Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: ERCC1, Antibody; Anti-ERCC1 Antibody Picoband; anti-ERCC1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (varies by lot)

• Applicable Applications for anti-ERCC1 antibody: ELISA, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
• Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
• Immunogen: E Coli-derived human ERCC1 recombinant protein (Position: R106-E291).
• Preparation and Storage: Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.

FCM/FACS (Flow Cytometry)

(Figure 3. Flow Cytometry analysis of HL-60 cells using anti-ERCC1 antibody (AAA126016).Overlay histogram showing HL-60 cells stained with AAA126016 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ERCC1 Antibody (AAA126016, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence)

(Figure 2. IF analysis of ERCC1 using anti-ERCC1 antibody (AAA126016).ERCC1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-ERCC1 Antibody (AAA126016) overnight at 4 degree C. DyLight594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

WB (Western Blot)

(Figure 1. Western blot analysis of ERCC1 using anti-ERCC1 antibody (AAA126016).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human HepG2 whole cell lysates,Lane 3: human COLO-320 whole cell lysates,Lane 4: rat kidney tissue lysates,Lane 5: mouse kidney tissue lysates,Lane 6: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERCC1 antigen affinity purified polyclonal antibody (#AAA126016) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ERCC1 at approximately 33 kDa. The expected band size for ERCC1 is at 33 kDa.)

Related Product Information for anti-ERCC1 antibody

DNA excision repair protein ERCC-1 is a protein that in humans is encoded by the ERCC1 gene. The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand.

Product Categories/Family for anti-ERCC1 antibody

Primary Antibodies, Rabbit Polyclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 260593725
• NCBI GeneID: 2067
• NCBI Accession #: NP_001159521.1
• NCBI GenBank Nucleotide #: NM_001166049.1
• UniProt Accession #: P07992; Q7Z7F5; Q96S40
• Molecular Weight: 32,562 Da
• NCBI Official Full Name: DNA excision repair protein ERCC-1 isoform 3
• NCBI Official Synonym Full Names: excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)
• NCBI Official Symbol: ERCC1
• NCBI Official Synonym Symbols: UV20; COFS4; RAD10
• NCBI Protein Information: DNA excision repair protein ERCC-1
• UniProt Protein Name: DNA excision repair protein ERCC-1
• UniProt Gene Name: ERCC1
• UniProt Entry Name: ERCC1_HUMAN