IF (Immunofluorescence)
(Immunofluorescence analysis of Jurkat cells (treated with PMA) and Jurkat cells (untreated), using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AAA283116) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
Rabbit anti-Human ERK1-T202/Y204 + ERK2-T185/Y187 Monoclonal Antibody | anti-MAPK1/MAPK3 antibody
Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb
IF (Immunofluorescence)
(Immunofluorescence analysis of Jurkat cells (treated with PMA) and Jurkat cells (untreated), using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AAA283116) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of Jurkat cells (treated with PMA) and Jurkat cells (untreated), using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AAA283116) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded Rat fallopian tube using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AAA283116) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded Rat fallopian tube using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AAA283116) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemisry)
(Immunohistochemistry analysis of paraffin-embedded Rat brain using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AAA283116) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemisry)
(Immunohistochemistry analysis of paraffin-embedded Rat brain using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AAA283116) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
WB (Western Blot)
(Western blot analysis of lysates from C6 cells using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AAA283116) at 1:1000 dilution (upper) or ERK1/2 Rabbit mAb (A4782) at1:3000 dilution (lower) incubated overnight at 4?. C6 cells were treated by PMA (200 nM) at 37? for 10 minutes after serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 30 ug per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 45s.)
WB (Western Blot)
(Western blot analysis of lysates from C6 cells using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AAA283116) at 1:1000 dilution (upper) or ERK1/2 Rabbit mAb (A4782) at1:3000 dilution (lower) incubated overnight at 4?. C6 cells were treated by PMA (200 nM) at 37? for 10 minutes after serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 30 ug per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 45s.)
WB (Western Blot)
(Western blot analysis of lysates from 293T cells using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AAA283116) at 1:1000 dilution (upper) or ERK1/2 Rabbit mAb (A4782) at1:3000 dilution (lower) incubated overnight at 4?. 293T cells were treated by PMA (100 nM) at 37? for 30 minutes after serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 30 ug per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
WB (Western Blot)
(Western blot analysis of lysates from 293T cells using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AAA283116) at 1:1000 dilution (upper) or ERK1/2 Rabbit mAb (A4782) at1:3000 dilution (lower) incubated overnight at 4?. 293T cells were treated by PMA (100 nM) at 37? for 30 minutes after serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 30 ug per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
NCBI and Uniprot Product Information
Observed MW: 42kDa/44kDa
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Product Notes
The MAPK1/MAPK3 mapk3 (Catalog #AAA283116) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ERK1-T202/Y204 + ERK2-T185/Y187 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the MAPK1/MAPK3 mapk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: LTEYV. It is sometimes possible for the material contained within the vial of "ERK1-T202/Y204 + ERK2-T185/Y187, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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