FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA279119 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
MAPK1/MAPK3 recombinant antibody
MAPK1/MAPK3 Recombinant Monoclonal Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot, ELISA
Purity
Affinity purified
Synonyms
MAPK1/MAPK3, Recombinant Antibody; MAPK1/MAPK3 Recombinant Monoclonal Antibody; Mitogen-activated protein kinase 3 (MAP kinase 3)(MAPK 3)(EC 2.7.11.24)(ERT2)(Extracellular signal-regulated kinase 1)(ERK-1)(Insulin-stimulated MAP2 kinase)(MAP kinase isoform p44)(p44-MAPK)(Microtubule-associated protein 2 kinase)(p44-ERK1); MAPK3; ERK1 PRKM3; Mitogen-activated protein kinase 1 (MAP kinase 1)(MAPK 1)(EC 2.7.11.24)(ERT1)(Extracellular signal-regulated kinase 2)(ERK-2)(MAP kinase isoform p42)(p42-MAPK)(Mitogen-activated protein kinase 2)(MAP kinase 2)(MAPK 2); MAPK1; ERK2 PRKM1 PRKM2; MAPK1/MAPK3 recombinant antibody
Host
HEK293F Cell
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
29B10
Purity/Purification
Affinity purified
Form/Format
Liquid; PBS, 150mM NaCl, 0.02% sodium azide, 50% glycerol, pH 7.4.
Applicable Applications for MAPK1/MAPK3 recombinant antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen Species
Human
Immunogen
A synthesized peptide derived from Human MAPK1/MAPK3
Conjugation
Non-conjugated
Research Area
Others
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA279119 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA279119 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
IHC (Immunohiostchemistry)
(IHC image of AAA279119 diluted at 1:50 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.64% DAB.)
IHC (Immunohiostchemistry)
(IHC image of AAA279119 diluted at 1:50 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.64% DAB.)
WB (Western Blot)
(Western BlotPositive WB detected in: THP-1 whole cell lysate, HEPG2 whole cell lysate,MCF7 whole cell lysate,293 whole cell lysate,K562 whole cell lysate,PC-3 whole cell lysate, Mouse brain tissue lysateAll lanes: ERK1/2 antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 42,44 kDaObserved band size: 42,44 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: THP-1 whole cell lysate, HEPG2 whole cell lysate,MCF7 whole cell lysate,293 whole cell lysate,K562 whole cell lysate,PC-3 whole cell lysate, Mouse brain tissue lysateAll lanes: ERK1/2 antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 42,44 kDaObserved band size: 42,44 kDa)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,136 Da
NCBI Official Full Name
mitogen-activated protein kinase 3 isoform 2
NCBI Official Synonym Full Names
mitogen-activated protein kinase 3
NCBI Official Symbol
MAPK3
NCBI Official Synonym Symbols
ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
NCBI Protein Information
mitogen-activated protein kinase 3; MAPK 1; MAP kinase isoform p44; insulin-stimulated MAP2 kinase; extracellular signal-related kinase 1; extracellular signal-regulated kinase 1; microtubule-associated protein 2 kinase
UniProt Protein Name
Mitogen-activated protein kinase 3
UniProt Gene Name
MAPK3
UniProt Synonym Gene Names
ERK1; PRKM3; MAP kinase 3; MAPK 3; ERK-1; p44-MAPK
UniProt Entry Name
MK03_HUMAN
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Product Notes
The MAPK1/MAPK3 mapk3 (Catalog #AAA279119) is a Recombinant Antibody produced from HEK293F Cell and is intended for research purposes only. The product is available for immediate purchase. The MAPK1/MAPK3 Recombinant Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MAPK1/MAPK3 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the MAPK1/MAPK3 mapk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAPK1/MAPK3, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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