Mouse EWSR1 Monoclonal Antibody | anti-EWSR1 antibody

EWSR1

Cat. Num. AAA76627

• Gene Names: EWSR1; EWS; bK984G1.4
• Reactivity: Human, Mouse, Rat, Dog, Equine
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot, Immunocytochemistry
• Synonyms: EWSR1, Antibody; EWSR1; anti-EWSR1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Dog, Equine
• Clonality: Monoclonal
• Isotype: Mouse IgG
• Form/Format: Purified liquid antibody in PBS, 50% glycerol plus 5mM of Sodium Azide.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-EWSR1 antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ICC (Immunocytochemistry)
• Immunogen: Full length human EWS expressed in and purified from E Coli.
• Preparation and Storage: Antibody can also be aliquotted and stored frozen at -20 degree C in a manual defrost freezer for six months without detectable loss of activity. The antibody can be stored at 2-8 degree C for 1 month without detectable loss ofactivity. Avoid repeated freeze-thaw cycles.

WB (Western Blot)

(Western blot analysis of different cell lines lysates using mouse mAB to EWS, MO22168, dilution 1:1,000 in green: [1] protein standard (red), [2] HeLa, [3] HEK293, [4] mouse NIH-3T3, [5] rat PC12, [6] equine NBL6 and [7] canine A72 cells. The strong band at ~80kDa corresponds to the EWS protein seen in all species tested.)

Application Data

(An image of a HeLa cell cultures stained with antibody MO22168 (green) to EWS and counterstained with chicken antibody to vimentin (red). Blue is the Hoechst DNA stain. The EWS protein is clearly localized along with the DNA in the nucleus.)

Related Product Information for anti-EWSR1 antibody

The Ewing Sarcoma breakpoint region 1 gene EWSR1, was discovered as the name suggests as it is located at the breakpoint on human chromosome 22 which may becomes fused to segments of other chromosomes following chromoplexy, a burst of complex chromosomal rearrangement seen in cancer cells. The genetic rearrangement produces a set of aberrant genes consisting of the 5? of the EWSR1 gene fused to gene segments of several different transcriptional regulator proteins. The normal EWSR1 gene encodes a protein, EWS RNA binding protein 1, containing an N-terminal transactivation domain followed by a single RRM domain and a single Zinc Finger domain of the ZnF RBZ type. Chromoplexy results in the production of aberrant genes encoding the N-terminal EWS transactivation domain fused to DNA binding segments of various transcription factors, resulting in strong activation of transcription. EWS is an abundant, ubiquitous and multifunctional protein involved in regulating gene expression, cell division, RNA processing and transport. EWS is localized primarily in the nucleus of cells, but has also been found in the cytoplasm, and associated with the plasma membrane in a fashion regulated by the protein kinase PYK2.

NCBI and Uniprot Product Information

• NCBI GI #: 253970502
• NCBI GeneID: 2130
• NCBI Accession #: NP_001156757.1
• NCBI GenBank Nucleotide #: NM_001163285.1
• UniProt Accession #: Q01844; Q5THL0; Q92635; Q96FE8; Q96MN4; Q96MX4; Q9BWA2; B0QYK1
• Molecular Weight: 68,478 Da
• NCBI Official Full Name: RNA-binding protein EWS isoform 3
• NCBI Official Synonym Full Names: EWS RNA-binding protein 1
• NCBI Official Symbol: EWSR1
• NCBI Official Synonym Symbols: EWS; bK984G1.4
• NCBI Protein Information: RNA-binding protein EWS; Ewing sarcoma breakpoint region 1; Ewings sarcoma EWS-Fli1 (type 1) oncogene
• UniProt Protein Name: RNA-binding protein EWS
• UniProt Gene Name: EWSR1
• UniProt Synonym Gene Names: EWS
• UniProt Entry Name: EWS_HUMAN