IP (Immunoprecipitation)
(EZR was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate0.5 uL anti-EZR mouse monoclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-EZR mouse monoclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 69 kDaObserved band size: 90 kDa)
Mouse anti-Human Ezrin Monoclonal Antibody | anti-EZR antibody
Anti-Ezrin Antibody, Mouse Monoclonal
Gene Names
EZR; CVL; CVIL; VIL2
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A
Synonyms
Ezrin, Antibody; Anti-Ezrin Antibody, Mouse Monoclonal; Ezrin/villin 2/EZR Antibody, Mouse MAb; ezrin; Anti-CVIL Antibody; Anti-CVL Antibody; Anti-HEL-S-105 Antibody; Anti-VIL2 Antibody; anti-EZR antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
02
Specificity
Human Ezrin/villin 2/EZR
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-EZR antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Recombinant Human Ezrin/villin 2/EZR Protein
Conjugation
Unconjugated
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Ezrin/villin 2/EZR (rh Ezrin/villin 2/EZR). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(EZR was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate0.5 uL anti-EZR mouse monoclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-EZR mouse monoclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 69 kDaObserved band size: 90 kDa)
IP (Immunoprecipitation)
(EZR was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate0.5 uL anti-EZR mouse monoclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-EZR mouse monoclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 69 kDaObserved band size: 90 kDa)
WB (Western Blot)
(Anti-EZR mouse monoclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:69 kDaObserved band size:90 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-EZR mouse monoclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:69 kDaObserved band size:90 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-EZR antibody
VIL2, also known as villin-2, belongs to the ERM protein family. It is expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. VIL2 contains 1 FERM domain and may be involved in connections of major cytoskel
Product Categories/Family for anti-EZR antibody
References
Grnholm M, et al. (1999) Homotypic and heterotypic interaction of the neurofibromatosis 2 tumor suppressor protein merlin and the ERM protein ezrin. J Cell Sci. 112(6):895-904.Sitaraman, et al. (2002) The adenosine 2b receptor is recruited to the plasma membrane and associates with E3KARP and Ezrin upon agonist stimulation. J Biol Chem. 277(36):33188-95.Gould KL, et al. (1989) cDNA cloning and sequencing of the protein-tyrosine kinase substrate, ezrin, reveals homology to band 4.1. EMBO J. 8(13):4133-42.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
69,413 Da
NCBI Official Full Name
ezrin
NCBI Official Synonym Full Names
ezrin
NCBI Official Symbol
EZR
NCBI Official Synonym Symbols
CVL; CVIL; VIL2
NCBI Protein Information
ezrin; p81; villin-2; cytovillin 2; villin 2 (ezrin)
UniProt Protein Name
Ezrin
UniProt Gene Name
EZR
UniProt Synonym Gene Names
VIL2
UniProt Entry Name
EZRI_HUMAN
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Product Notes
The EZR ezr (Catalog #AAA255611) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Ezrin Antibody, Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Ezrin can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the EZR ezr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ezrin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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