Rat F4/80 Monoclonal Antibody | anti-F4/80 antibody

RAT ANTI MOUSE F4/80

Cat. Num. AAA12225

• Gene Names: Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
• Applications: Flow Cytometry, Immunohistochemistry, Immunohistochemistry, Immunohistochemistry, Immunoprecipitation, Immunofluorescence, immunoassay, Radioimmunoassay
• Synonyms: F4/80, Antibody; RAT ANTI MOUSE F4/80; anti-F4/80 antibody

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: Cl:A3-1
• Form/Format: Tissue Culture Supernatant - liquid
• Sequence Length: 931

• Applicable Applications for anti-F4/80 antibody: Flow Cytometry (FC), Immunohistology Frozen, Immunohistology Paraffin*, Immunohistology Resin (RE), Immunoprecipitation (IP), Immunofluorescence (IF), Radioimmunoassays (RIA), Immunoelectron Microscopy (EM)
• Application Notes: FC: Neat- 1/10; Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul ; ; Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls; ¹ Rat anti Mouse F4/80 antibody, clone A3-1 requires pre-treatment of paraffin sections prior to staining. Proteinase K is recommended for tissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixed for more than 24 hours. Please view the protocol at Antigen Retrieval Techniques.
• Immunogen: Thioglycollate stimulated peritoneal macrophages from C57BL/6 mice.
• Perservative Stabilisers: 0.09% Sodium Azide
• Preparation: Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
• Fusion Partners: Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS1 myeloma cell line.
• Buffer Solution: Phosphate buffered saline
• Target Species: Mouse
• Preparation and Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.; Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

Application Data

(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)

Application Data

(Published customer image: Post-injury 7,8-dihydroxyflavone treatment increased brain BDNF levels and promoted CREB activation. (A) Bar graphs demonstrating brain derived neurotrophic factor (BDNF) protein concentrations measured by enzyme-linked immunosorbent assay (ELISA) in sham-injured, vehicle-treated, and 20 mg/kg 7,8-dihydroxyflavone (DHF 20)-treated mice at 4 days post-TBI. DHF 20 significantly increased BDNF protein levels in the ipsilateral hemisphere at 4 days post-injury. (B) Bar graphs demonstrating BDNF mRNA expression measured by real -time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) at 1 and 4 days post-injury. DHF 20 significantly increased BDNF mRNA levels in the ipsilateral hemisphere at 4 days post-injury. (C) Western blot analysis of the phospho-CREB level in the ipsilateral hemisphere of sham-injured, vehicle-treated, and DHF 20-treated mice at 1 h, 1 day, and 4 days post-injury. DHF 20 enhanced CREB phosphorylation at 4 days post-injury. (D) Identification of phospho-CREB-positive cells 4 day post-injury in the peri-contusional margin by double immunofluorescence staining. Phospho-CREB is shown in red, and NeuN (neurons), GFAP (astrocytes), or F4/80 (microglia) is shown in green. Co-localization of phospho-CREB with NeuN is shown by yellow labeling. Sections were stained with DAPI (blue) to show all nuclei. Values are mean +/- SEM; *P)

Application Data

(Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: Pacific Blue(AAA12225PB))

Application Data

(Staining of J774 cells with Rat anti Mouse F4/80 antigen:Biotin)

Application Data

(Frozen mouse spleen stained with A: Rat anti Mouse F4/80 followed by Goat anti Rat IgG:HRP or B: Rat anti Mouse F4/80 preincubated with 2 molar excess of Human anti Idiotypic AAA12225 (HCA154) followed by Goat anti Rat IgG:HRP)

Application Data

(Staining of J774 cells with Rat anti Mouse F4/80 antigen:Low Endotoxin)

Application Data

(Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: RPE - Alexa Fluor 750 (AAA12225P750))

Application Data

(Staining of mouse peritoneal macrophages cells with Rat anti Mouse F4/80 antigen:APC)

Application Data

(Immunofluorescence image of mouse small intestine stained with Rat anti Mouse F4/80 , red and Rat anti Mouse CD45 , green; nuclei are stained with DAPI, blue. The image higlights macrophages within the lamina propria)

Application Data

(Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen:FITC)

Related Product Information for anti-F4/80 antibody

Description: Rat anti Mouse F4/80 antibody, clone A3-1 recognizes the murine F4/80 antigen, a ~160 kDa cell surface glycoprotein member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1).

Expression of F4/80 is heterogeneous and is modulated during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans cells, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen (Hume, et al. 1984). F4/80 antigen is also expressed on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node (Gordon, et al. 1994). The ligands and biological functions of the F4/80 antigen have not been fully determined but a role for F4/80 in the generation of efferent CD8+ve regulatory T cells is proposed (Lin, et al. 2005)

Rat anti mouse F4/80 antibody, clone Cl:A3-1 modulates cytokine levels released in response to Listeria monocytogenes (Warschkau & Kiderlen, 1999).

A Human anti-idiotypic CI:A31 antibody, clone 17867 (HCA154 ) which binds to and blocks activity of Rat anti mouse F4/80 antibody, clone Cl:A3-1 is also available for use as a control in experiments utilizing clone A3-1.

NCBI and Uniprot Product Information

• NCBI GI #: 33859546
• NCBI GeneID: 13733
• NCBI Accession #: NP_034260.1
• NCBI GenBank Nucleotide #: NM_010130.4
• NCBI Official Full Name: EGF-like module-containing mucin-like hormone receptor-like 1
• NCBI Official Synonym Full Names: EGF-like module containing, mucin-like, hormone receptor-like sequence 1
• NCBI Official Symbol: Emr1
• NCBI Official Synonym Symbols: Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
• NCBI Protein Information: EGF-like module-containing mucin-like hormone receptor-like 1; EGF-like module receptor 1; EMR1 hormone receptor; cell surface glycoprotein F4/80; lymphocyte antigen 71
• UniProt Protein Name: EGF-like module-containing mucin-like hormone receptor-like 1
• UniProt Gene Name: Emr1
• UniProt Synonym Gene Names: Gpf480
• UniProt Entry Name: EMR1_MOUSE