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FCM/FACS (Flow Cytometry)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant FH protein (red) or empty vector (blue) stained using FH antibody)
Mouse FH Monoclonal Antibody | anti-FH antibody
FH antibody
Gene Names
FH; MCL; FMRD; LRCC; HLRCC; MCUL1
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
FH antibody was purified by affinity chromatography.
Synonyms
FH, Antibody; FH antibody; Monoclonal FH; Anti-FH; Fumarate Hydratase; Fumarase; HLRCC; LRCC; MCul1; anti-FH antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1F10
Purity/Purification
FH antibody was purified by affinity chromatography.
Form/Format
Liquid in PBS, pH 7.3, with 1% BSA, 50% glycerol and 0.02% NaN3
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
510
Applicable Applications for anti-FH antibody
WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence)
Biological Significance
FH is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer.
Cross-Reactivity
Human, Dog, Rat
Immunogen
Full length human recombinant protein of human FH (NP_000134) produced in HEK293T cell
Preparation and Storage
Store at 4°C short term, -20°C long term. Avoid repeated freeze/thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant FH protein (red) or empty vector (blue) stained using FH antibody)
FCM/FACS (Flow Cytometry)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant FH protein (red) or empty vector (blue) stained using FH antibody)
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant FH protein using FH antibody)
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant FH protein using FH antibody)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of FH protein in paraffin embedded Human Kidney tissue using FH antibody)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of FH protein in paraffin embedded Human Kidney tissue using FH antibody)
WB (Western Blot)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant FH protein (Right) or empty vector (Left) detected with FH antibody)
WB (Western Blot)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant FH protein (Right) or empty vector (Left) detected with FH antibody)
Related Product Information for anti-FH antibody
Mouse monoclonal FH antibody
Product Categories/Family for anti-FH antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
fumarate hydratase, mitochondrial
NCBI Official Synonym Full Names
fumarate hydratase
NCBI Official Symbol
FH
NCBI Official Synonym Symbols
MCL; FMRD; LRCC; HLRCC; MCUL1
NCBI Protein Information
fumarate hydratase, mitochondrial
UniProt Protein Name
Fumarate hydratase, mitochondrial
UniProt Gene Name
FH
UniProt Synonym Gene Names
Fumarase
UniProt Entry Name
FUMH_HUMAN
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Product Notes
The FH fh (Catalog #AAA106545) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's FH can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the FH fh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FH, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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