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WB (Western Blot)
(Detection of human Fibroblast Activation Protein alpha/FAP by western blot. Samples: Whole cell lysate (50 ug) from SNB-75, HeLa, IMR-90, HEK293T, and U2OS cells prepared using NETN lysis buffer. Antibody: Rabbit anti-FAP recombinant monoclonal antibody (AAA213608 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
Fibroblast Activation Protein alpha/FAP Recombinant Antibody | FAP recombinant antibody
Rabbit anti-Fibroblast Activation Protein alpha/FAP Recombinant Monoclonal Antibody [BLR150J]
Gene Names
FAP; FAPA; DPPIV
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
Fibroblast Activation Protein alpha/FAP, Recombinant Antibody; Rabbit anti-Fibroblast Activation Protein alpha/FAP Recombinant Monoclonal Antibody [BLR150J]; post-proline cleaving enzyme; SIMP; Serine integral membrane protease; integral membrane serine protease; prolyl endopeptidase FAP; surface-expressed protease; Fibroblast activation protein alpha; FAPalpha; FAPA; DPPIV; dipeptidyl peptidase FAP; 170 kDa melanoma membrane-bound gelatinase; gelatine degradation protease FAP; seprase; Prolyl endopeptidase FAP; fibroblast activation protein alpha; FAP recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR150J
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
250ug/ml (varies by lot)
Applicable Applications for FAP recombinant antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Residues 26-760 (ECD)
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Fibroblast Activation Protein alpha/FAP by western blot. Samples: Whole cell lysate (50 ug) from SNB-75, HeLa, IMR-90, HEK293T, and U2OS cells prepared using NETN lysis buffer. Antibody: Rabbit anti-FAP recombinant monoclonal antibody (AAA213608 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of human Fibroblast Activation Protein alpha/FAP by western blot. Samples: Whole cell lysate (50 ug) from SNB-75, HeLa, IMR-90, HEK293T, and U2OS cells prepared using NETN lysis buffer. Antibody: Rabbit anti-FAP recombinant monoclonal antibody (AAA213608 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation)
(Detection of human Fibroblast Activation Protein alpha/FAP by western blot of immunoprecipitates. Samples: Whole cell lysate (500 ug per IP reaction; 5% of IP loaded) from IMR-90 cells. Antibody: Rabbit anti-FAP recombinant monoclonal antibody (AAA213608 lot 1) used for IP at 20 ul/mg lysate. For blotting immunoprecipitated FAP, AAA213608 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human Fibroblast Activation Protein alpha/FAP by western blot of immunoprecipitates. Samples: Whole cell lysate (500 ug per IP reaction; 5% of IP loaded) from IMR-90 cells. Antibody: Rabbit anti-FAP recombinant monoclonal antibody (AAA213608 lot 1) used for IP at 20 ul/mg lysate. For blotting immunoprecipitated FAP, AAA213608 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohiostchemistry)
(Detection of human Fibroblast Activation Protein alpha/FAP FFPE human breast carcinoma by immunohistochemistry. Antibody: Rabbit anti-Fibroblanst Activation Protein alpha/FAP recombinant monoclonal antibody (AAA213608 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohiostchemistry)
(Detection of human Fibroblast Activation Protein alpha/FAP FFPE human breast carcinoma by immunohistochemistry. Antibody: Rabbit anti-Fibroblanst Activation Protein alpha/FAP recombinant monoclonal antibody (AAA213608 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human Fibroblast Activation Protein alpha/FAP in FFPE human BJ cells by immunocytochemistry. Antibody: Rabbit anti-Fibroblanst Activation Protein alpha/FAP recombinant monoclonal antibody (AAA213608 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human Fibroblast Activation Protein alpha/FAP in FFPE human BJ cells by immunocytochemistry. Antibody: Rabbit anti-Fibroblanst Activation Protein alpha/FAP recombinant monoclonal antibody (AAA213608 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
Related Product Information for FAP recombinant antibody
Fibroblast Activation Protein alpha, or FAP, is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. FAP is involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. [taken from NCBI Entrez Gene (Gene ID: 2191)].
Product Categories/Family for FAP recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
87,713 Da
NCBI Official Full Name
seprase
NCBI Official Synonym Full Names
fibroblast activation protein, alpha
NCBI Official Symbol
FAP
NCBI Official Synonym Symbols
FAPA; DPPIV
NCBI Protein Information
seprase; integral membrane serine protease; 170 kDa melanoma membrane-bound gelatinase
UniProt Protein Name
Seprase
UniProt Gene Name
FAP
UniProt Entry Name
SEPR_HUMAN
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Product Notes
The FAP fap (Catalog #AAA213608) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Fibroblast Activation Protein alpha/FAP Recombinant Monoclonal Antibody [BLR150J] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Fibroblast Activation Protein alpha/FAP can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the FAP fap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fibroblast Activation Protein alpha/FAP, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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