Mouse FOXA1/HNF3A Monoclonal Antibody | anti-FOXA1 antibody

FOXA1/HNF3A

Cat. Num. AAA214382

• Gene Names: FOXA1; HNF3A; TCF3A
• Reactivity: Human, Rat. Others not known.
• Applications: Immunohistochemistry
• Synonyms: FOXA1/HNF3A, Antibody; FOXA1/HNF3A; Forkhead box protein A1 (FOXA1); Hepatocyte nuclear factor 3-alpha (HNF-3-alpha or HNF-3A or HNF3A); Transcription factor 3A (TCF-3A or TCF3A); anti-FOXA1 antibody

• Host: Mouse
• Reactivity: Human, Rat. Others not known.
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: rFOXA1/1515
• Specificity: The transcription factor Forkhead-box A1 (FOXA1), also known as hepatocyte nuclear factor 3-alpha, is a member of the FOX class of transcription factors. HNF-1 (? and ?), HNF-3 (?, ? and ?), HNF-4 (? and ?), and HNF-6 compose, in part, a homeoprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. FOXA1 is expressed in normal breast ductal epithelium and other epithelium in different organs, such as lung, pancreas, bladder, prostate, and colon. Recently, FOXA1 has been shown to be a major determinant of estrogen-ER activity and endocrine response in breast cancer cells. FOXA1 expression correlates with estrogen receptor (ER)-positivity, especially in luminal subtype A breast cancers, which is associated with favorable prognosis. FOXA1 is useful in the sub-classification of breast carcinomas.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 472

• Applicable Applications for anti-FOXA1 antibody: IHC (Immunohistochemistry)
• Immunogen: Recombinant fragment of human FOXA1 protein (around aa 372-472)
• Cellular Localization: Nuclear
• Hu-chromosome Location: 14q21.1
• Positive Control: HepG-2 or MCF-7 cells. Liver, Pancreas, Bladder, Prostate, Colon and Lung and Breast.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis of Purified FOXA1 Mouse Recombinant Monoclonal Antibody (rFOXA1/1515).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with FOXA1 Mouse Recombinant Monoclonal Antibody (rFOXA1/1515).)

References

Andre Albergaria, Joana Paredes, Barbara Sousa, Fernanda Milanezi, Vitor Carneiro, Joana Bastos, Sandra Costa, Daniella Vieira, Nair Lopes, Eric W Lam, Nuno Lunet andFernando Schmitt. Expression of FOXA1 and GATA-3 in breast cancer: the prognostic significance in hormone receptor-negative tumours. Breast Cancer Research2009, 11:R40.

NCBI and Uniprot Product Information

• NCBI GI #: 24497501
• NCBI GeneID: 3169
• NCBI Accession #: NP_004487.2
• NCBI GenBank Nucleotide #: NM_004496.3
• UniProt Accession #: P55317; Q9H2A0; B2R9H6; B7ZAP5
• Molecular Weight: 49kDa
• NCBI Official Full Name: hepatocyte nuclear factor 3-alpha
• NCBI Official Synonym Full Names: forkhead box A1
• NCBI Official Symbol: FOXA1
• NCBI Official Synonym Symbols: HNF3A; TCF3A
• NCBI Protein Information: hepatocyte nuclear factor 3-alpha
• UniProt Protein Name: Hepatocyte nuclear factor 3-alpha
• UniProt Gene Name: FOXA1
• UniProt Synonym Gene Names: HNF3A; TCF3A; HNF-3-alpha; HNF-3A; TCF-3A