<
IP (Immunoprecipitation)
(Immunoprecipitating FOXA1 in Hela whole cell lysateLane 1: Rabbit control IgG instead of in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: Hela whole cell lysate?500ug?Lane 3: Hela whole cell lysate (10ug))
FOXA1 recombinant antibody
FOXA1 Antibody
Gene Names
FOXA1; HNF3A; TCF3A
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot, ELISA
Purity
Affinity-chromatography
Synonyms
FOXA1, Recombinant Antibody; FOXA1 Antibody; Hepatocyte nuclear factor 3-alpha (HNF-3-alpha) (HNF-3A) (Forkhead box protein A1) (Transcription factor 3A) (TCF-3A); FOXA1; HNF3A TCF3A; FOXA1 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
3B2
Purity/Purification
Affinity-chromatography
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, PH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applicable Applications for FOXA1 recombinant antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Antibody Type
Recombinant Antibody
Conjugation
Non-conjugated
Immunogen
A synthesized peptide derived from human FOXA1
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
IP (Immunoprecipitation)
(Immunoprecipitating FOXA1 in Hela whole cell lysateLane 1: Rabbit control IgG instead of in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: Hela whole cell lysate?500ug?Lane 3: Hela whole cell lysate (10ug))
IP (Immunoprecipitation)
(Immunoprecipitating FOXA1 in Hela whole cell lysateLane 1: Rabbit control IgG instead of in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: Hela whole cell lysate?500ug?Lane 3: Hela whole cell lysate (10ug))
IHC (Immunohistochemisry)
(IHC image diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohistochemisry)
(IHC image diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohiostchemistry)
(IHC image diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohiostchemistry)
(IHC image diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
WB (Western Blot)
(Western BlotPositive WB detected in: MCF-7 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, Hela whole cell lysateAll lanes: FOXA1 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 50, 46 kDaObserved band size: 50 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: MCF-7 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, Hela whole cell lysateAll lanes: FOXA1 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 50, 46 kDaObserved band size: 50 kDa)
Related Product Information for FOXA1 recombinant antibody
Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promoter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.
Product Categories/Family for FOXA1 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52 kDa
NCBI Official Full Name
hepatocyte nuclear factor 3-alpha
NCBI Official Synonym Full Names
forkhead box A1
NCBI Official Symbol
FOXA1
NCBI Official Synonym Symbols
HNF3A; TCF3A
NCBI Protein Information
hepatocyte nuclear factor 3-alpha; HNF-3A; TCF-3A; HNF-3-alpha; forkhead box protein A1; transcription factor 3A
UniProt Protein Name
Hepatocyte nuclear factor 3-alpha
UniProt Gene Name
FOXA1
UniProt Synonym Gene Names
HNF3A; TCF3A; HNF-3-alpha; HNF-3A; TCF-3A
UniProt Entry Name
FOXA1_HUMAN
Similar Products
Product Notes
The FOXA1 foxa1 (Catalog #AAA243867) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FOXA1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FOXA1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the FOXA1 foxa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FOXA1, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.