FCM/FACS (Flow Cytometry)
(Fig-3: Intracellular flow analysis of FSH receptor antibody on HeLa cells using 0.5 ug/10^6 cells (Clone: ABM5D29). Green represents isotype control; red represents anti-FSH receptor antibody. Goat anti-mouse PE conjugate was used as secondary. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).)
Mouse anti-Human FSH Receptor Monoclonal Antibody | anti-FSHR antibody
Monoclonal Antibody to FSH Receptor (Clone: ABM5D29)
Gene Names
FSHR; LGR1; ODG1; FSHR1; FSHRO
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
PurifiedProtein G Chromatography
Synonyms
FSH Receptor, Antibody; Monoclonal Antibody to FSH Receptor (Clone: ABM5D29); Follicle-stimulating hormone receptor; FSH-R; Follitropin receptor; anti-FSHR antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, Kappa
Clone Number
ABM5D29
Purity/Purification
Purified
Protein G Chromatography
Protein G Chromatography
Form/Format
25 ug in 50 uL/100 ug in 200 uL PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Sequence Length
695
Applicable Applications for anti-FSHR antibody
FCM/FACS (Flow Cytometry), WB (Western Blot)
Antigen
FSH Receptor
Immunogen Information
A partial length recombinant protein (a.a 03-200) of FSH Receptor was used as the immunogen for this antibody.
Conjugation
Unconjugated
Preparation and Storage
Store the antibody at 4°C; stable for 6 months.
For long-term storage; store at -20°C.
Avoid repeated freeze and thaw cycles.
For long-term storage; store at -20°C.
Avoid repeated freeze and thaw cycles.
FCM/FACS (Flow Cytometry)
(Fig-3: Intracellular flow analysis of FSH receptor antibody on HeLa cells using 0.5 ug/10^6 cells (Clone: ABM5D29). Green represents isotype control; red represents anti-FSH receptor antibody. Goat anti-mouse PE conjugate was used as secondary. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).)
FCM/FACS (Flow Cytometry)
(Fig-3: Intracellular flow analysis of FSH receptor antibody on HeLa cells using 0.5 ug/10^6 cells (Clone: ABM5D29). Green represents isotype control; red represents anti-FSH receptor antibody. Goat anti-mouse PE conjugate was used as secondary. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).)
FCM/FACS (Flow Cytometry)
(Fig-2: Intracellular flow analysis of FSH receptor antibody on A431 cells using 0.5 ug/10^6 cells (Clone: ABM5D29). Green represents isotype control; red represents anti-FSH receptor antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).)
FCM/FACS (Flow Cytometry)
(Fig-2: Intracellular flow analysis of FSH receptor antibody on A431 cells using 0.5 ug/10^6 cells (Clone: ABM5D29). Green represents isotype control; red represents anti-FSH receptor antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).)
WB (Western Blot)
(Fig-1: Western blot analysis of FSH Receptor. Anti-FSH Receptor antibody (Clone: ABM5D29) was used at 2 ug/ml on Hela lysate.)
WB (Western Blot)
(Fig-1: Western blot analysis of FSH Receptor. Anti-FSH Receptor antibody (Clone: ABM5D29) was used at 2 ug/ml on Hela lysate.)
Related Product Information for anti-FSHR antibody
FSH receptor is a member of the G protein coupled receptor family consisting of 9 introns and 10 exons and a promoter region and is located on chromosome 2p21. FSH receptor was shown to be expressed selectively on the luminal surface of tumor blood vessels. A general characteristic of the blood vessels that express the endothelial FSH receptor is that they are located at the periphery of the tumors. Many studies showed that FSH receptor polymorphisms at positions 307 and 680 may be even more relevant for clinical practice for the reasons of regulating the ovarian reaction to hormone, controlling ovarian hyperstimulation, changing the menstrual cycle, and causing POF (Premature Ovarian Failure) and PCOS (Polycystic Ovary Syndrome).
Product Categories/Family for anti-FSHR antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
follicle-stimulating hormone receptor isoform 1
NCBI Official Synonym Full Names
follicle stimulating hormone receptor
NCBI Official Symbol
FSHR
NCBI Official Synonym Symbols
LGR1; ODG1; FSHR1; FSHRO
NCBI Protein Information
follicle-stimulating hormone receptor
UniProt Protein Name
Follicle-stimulating hormone receptor
UniProt Gene Name
FSHR
UniProt Synonym Gene Names
LGR1; FSH-R
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Product Notes
The FSHR fshr (Catalog #AAA78433) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to FSH Receptor (Clone: ABM5D29) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FSH Receptor can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the FSHR fshr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FSH Receptor, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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