IF (Immunofluorescence)
(ICC/IF analysis of GALE in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GALE antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Mouse anti-Human GALE Monoclonal Antibody | anti-GALE antibody
GALE antibody
Gene Names
GALE; SDR1E1
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
GALE, Antibody; GALE antibody; uDP-glucose 4-epimeraes; SDR1E1.uDP-glucose 4-epimeraes; uDP-galactose 4-epimerase; Galactowaldenase; FLJ95174; FLJ97302; anti-GALE antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT6G10
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
348
Applicable Applications for anti-GALE antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human GALE (1-348aa) purified from E. coli
Clone Note
Anti-human GALE mAb, clone AT6G10, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human GALE protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of GALE in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GALE antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IF (Immunofluorescence)
(ICC/IF analysis of GALE in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GALE antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GALE antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : MCF7 cell lysate Lane 2. : Jurkat cell lysate Lane 3. : A431 cell lysate Lane 4. : A549 cell lysate Lane 5. : HeLa cell lysate Lane 6. : HepG2 cell lysate)
WB (Western Blot)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GALE antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : MCF7 cell lysate Lane 2. : Jurkat cell lysate Lane 3. : A431 cell lysate Lane 4. : A549 cell lysate Lane 5. : HeLa cell lysate Lane 6. : HepG2 cell lysate)
Related Product Information for anti-GALE antibody
The enzyme UDP-glucose 4-epimerase, also known as UDP-galactose 4-epimerase or GALE, is a homodimeric epimerase found in bacterial, fungal, plant, and mammalian cells. This enzyme performs the final step in the Leloir pathway of galactose metabolism, catalyzing the reversible conversion of UDP-galactose to UDP-glucose. GALE tightly binds nicotinamide adenine dinucleotide (NAD+), a co-factor required for catalytic activity. Additionally, human and some bacterial GALE isoforms reversibly catalyze the formation of UDP-N-acetylgalactosamine (UDP-GalNAc) from UDP-N-acetylglucosamine (UDP-GlcNAc) in the presence of NAD+, an initial step in glycoprotein or glycolipid synthesis.
Product Categories/Family for anti-GALE antibody
References
Holden HM., et al. (2003) J. Biol. Chem. 278 (45): 43885-8.; Liu Y., et al. (1996) Biochemistry. 35 (23): 7615-20.; Thoden Jb., et al. (2001) J. Biol. Chem. 276 (18): 15131-6.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,180 Da
NCBI Official Full Name
UDP-glucose 4-epimerase
NCBI Official Synonym Full Names
UDP-galactose-4-epimerase
NCBI Official Symbol
GALE
NCBI Official Synonym Symbols
SDR1E1
NCBI Protein Information
UDP-glucose 4-epimerase
UniProt Protein Name
UDP-glucose 4-epimerase
UniProt Gene Name
GALE
UniProt Synonym Gene Names
UDP-GlcNAc 4-epimerase
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Product Notes
The GALE gale (Catalog #AAA48215) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GALE antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GALE can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the GALE gale for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GALE, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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