IP (Immunoprecipitation)
(Galectin1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg K562 Whole Cell LysateLane C:0.5 mg A431 Whole Cell LysateLane D:0.5 mg HL-60 Whole Cell Lysate2 uL anti-Galectin1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Galectin1 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 15 kDaObserved band size: 15 kDa)
Rabbit anti-Human Galectin-1 Monoclonal Antibody | anti-LGALS1 antibody
Recombinant Anti-Galectin-1 Antibody, Rabbit Monoclonal
Gene Names
LGALS1; GBP; GAL1
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A
Synonyms
Galectin-1, Antibody; Recombinant Anti-Galectin-1 Antibody, Rabbit Monoclonal; Galectin-1/LGALS1 Antibody, Rabbit MAb; lectin, galactoside-binding, soluble, 1; Anti-GAL1 Antibody; Anti-Galectin-1 Antibody; Anti-GBP Antibody; anti-LGALS1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
001
Specificity
Human Galectin-1/LGALS1
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-LGALS1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Human Galectin-1/LGALS1 protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human Galectin-1/LGALS1 (rh Galectin-1/LGALS1).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(Galectin1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg K562 Whole Cell LysateLane C:0.5 mg A431 Whole Cell LysateLane D:0.5 mg HL-60 Whole Cell Lysate2 uL anti-Galectin1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Galectin1 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 15 kDaObserved band size: 15 kDa)
IP (Immunoprecipitation)
(Galectin1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg K562 Whole Cell LysateLane C:0.5 mg A431 Whole Cell LysateLane D:0.5 mg HL-60 Whole Cell Lysate2 uL anti-Galectin1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Galectin1 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 15 kDaObserved band size: 15 kDa)
WB (Western Blot)
(Anti-Galectin1 rabbit monoclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: K562 Whole Cell LysateLane C: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:14 kDaObserved band size:14 kDa)
WB (Western Blot)
(Anti-Galectin1 rabbit monoclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: K562 Whole Cell LysateLane C: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:14 kDaObserved band size:14 kDa)
Related Product Information for anti-LGALS1 antibody
Galectin-1 (Gal-1, GAL1), is a member of the galectins, a family of animal lectins ranging from Caenorhabditis elegans to humans, which is defined by their affinity for beta-galactosides and by significant sequence similarity in the carbohydrate-binding site. It is a homodimer with a subunit molecular mass of 14.5 kDa, which contains six cysteine residues per subunit. The cysteine residues should be in a free state to maintain a molecular structure that is capable of showing lectin activity. This endogenous lectin widely expressed at sites of inflammation and tumor growth has been postulated as an attractive immunosuppressive agent to restore immune cell tolerance and homeostasis in autoimmune and inflammatory settings. On the other hand, galectin-1 contributes to different steps of tumor progression including cell adhesion, migration, and tumor-immune escape, suggesting that blockade of galectin-1 might result in therapeutic benefits in cancer. Several potential glycoprotein ligands for galectin-1 have been identified, including lysosome-associated membrane glycoproteins and fibronectin, laminin, as well as T-cell glycoproteins CD43 and CD45. Evidence points to Gal-1 and its ligands as one of the master regulators of such immune responses as T-cell homeostasis and survival, T-cell immune disorders, inflammation, and allergies as well as host-pathogen interactions.
References
Gaudet AD, et al. (2005) Expression and functions of galectin-1 in sensory and motoneurons. Curr Drug Targets. 6(4): 419-25.Kadoya T, et al. (2006) Structural and functional studies of galectin-1: a novel axonal regeneration-promoting activity for oxidized galectin-1. Curr Drug Targets. 6(4): 375-83.Camby I, et al. (2006) Galectin-1: a small protein with major functions. Glycobiology. 16(11): 137R-157R.Salatino M, et al. (2008) Galectin-1 as a potential therapeutic target in autoimmune disorders and cancer. Expert Opin Biol Ther. 8(1): 45-57.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14,716 Da
NCBI Official Full Name
galectin-1
NCBI Official Synonym Full Names
lectin, galactoside-binding, soluble, 1
NCBI Official Symbol
LGALS1
NCBI Official Synonym Symbols
GBP; GAL1
NCBI Protein Information
galectin-1; HBL; HPL; gal-1; HLBP14; galaptin; galectin 1; 14 kDa lectin; S-Lac lectin 1; lactose-binding lectin 1; 14 kDa laminin-binding protein; putative MAPK-activating protein PM12; beta-galactoside-binding lectin L-14-I; beta-galactoside-binding pro
UniProt Protein Name
Galectin-1
UniProt Gene Name
LGALS1
UniProt Synonym Gene Names
Gal-1; HLBP14
UniProt Entry Name
LEG1_HUMAN
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Product Notes
The LGALS1 lgals1 (Catalog #AAA254802) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-Galectin-1 Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Galectin-1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the LGALS1 lgals1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Galectin-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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