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FCM (Flow Cytometry)
(Flow cytometric analysis of K562 cells with GCNF antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
Mouse anti-Human GCNF Monoclonal Antibody | anti-GCNF antibody
GCNF Antibody
Gene Names
NR6A1; RTR; GCNF; NR61; hRTR; CT150; GCNF1; hGCNF
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
GCNF; Monoclonal Antibody; GCNF Antibody; GCNF 1 antibody; GCNF antibody; GCNF1 antibody; Germ cell nuclear factor antibody; hGCNF antibody; hRTR antibody; NR 61 antibody; NR61 antibody; nr6a1 antibody; NR6A1_HUMAN antibody; Nuclear receptor subfamily 6 group A member 1 antibody; Orphan nuclear receptor NR6A1 antibody; Retinoic acid receptor related testis associated receptor antibody; Retinoid receptor related testis specific receptor antibody; Retinoid receptor-related testis-specific receptor antibody; RTR antibody; anti-GCNF antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Clone Number
A10-G8
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-GCNF antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:1000
IHC: 1:100-1:500
ICC: 1:50-1:200
FC/FACS: 1:100-1:200
IHC: 1:100-1:500
ICC: 1:50-1:200
FC/FACS: 1:100-1:200
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of K562 cells with GCNF antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
FCM (Flow Cytometry)
(Flow cytometric analysis of K562 cells with GCNF antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
ICC (Immunocytochemistry)
(ICC staining GCNF (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining GCNF (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-GCNF antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-GCNF antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using anti-GCNF antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using anti-GCNF antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of GCNF on different cell lysates using anti-GCNF antibody at 1/1, 000 dilution. Positive control�� Lane1: K562 Lane2: NTERA-2 Lane3: HEK293 Lane4: HUVE-12 Lane5: HeLa)
WB (Western Blot)
(Western blot analysis of GCNF on different cell lysates using anti-GCNF antibody at 1/1, 000 dilution. Positive control�� Lane1: K562 Lane2: NTERA-2 Lane3: HEK293 Lane4: HUVE-12 Lane5: HeLa)
WB (Western Blot)
(Western blot analysis of GCNF on HEK293 (1) and GCNF -hIgGFc transfected HEK293 (2) cell lysate using anti-GCNF antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of GCNF on HEK293 (1) and GCNF -hIgGFc transfected HEK293 (2) cell lysate using anti-GCNF antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of GCNF on human GCNF recombinant protein using anti- GCNF antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of GCNF on human GCNF recombinant protein using anti- GCNF antibody at 1/1, 000 dilution.)
Related Product Information for anti-GCNF antibody
Germ cell nuclear factor (GCNF) is an orphan member of the nuclear receptor gene superfamily that influences neurogenesis and germ cell development. GCNF can homodimerize and bind DNA. GCNF regulates paracrine interaction between the oocyte and somatic cells by regulating the expression of BMP-15 and GDF-9, to affect female fertility. GCNF is present in spermatocytes and round spermatids of adult male mouse testis; northern blot and ribonuclease protection assays have shown GCNF is predominant in the testis. The gene expresses three alternatively spliced transcript variants.
Product Categories/Family for anti-GCNF antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,908 Da
NCBI Official Full Name
nuclear receptor subfamily 6 group A member 1 isoform 1
NCBI Official Synonym Full Names
nuclear receptor subfamily 6, group A, member 1
NCBI Official Symbol
NR6A1
NCBI Official Synonym Symbols
RTR; GCNF; NR61; hRTR; CT150; GCNF1; hGCNF
NCBI Protein Information
nuclear receptor subfamily 6 group A member 1; germ cell nuclear factor variant 1; retinoid receptor-related testis-specific receptor; retinoic acid receptor-related testis-associated receptor
UniProt Protein Name
Nuclear receptor subfamily 6 group A member 1
UniProt Gene Name
NR6A1
UniProt Synonym Gene Names
GCNF; GCNF; hGCNF; RTR; hRTR
UniProt Entry Name
NR6A1_HUMAN
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Product Notes
The GCNF nr6a1 (Catalog #AAA29911) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GCNF Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GCNF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:1000 IHC: 1:100-1:500 ICC: 1:50-1:200 FC/FACS: 1:100-1:200. Researchers should empirically determine the suitability of the GCNF nr6a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GCNF, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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