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product-image-AAA214651_WB7.jpg WB (Western Blot) (Western Blot Analysis of Human Brain, Mouse Brain, Rat Brain, Guinea Pig Brain and Hamster Brain tissue lysates using GFAP Mouse Monoclonal Antibody (GFAP/2076).)

Mouse anti-Human GFAP Monoclonal Antibody | anti-GFAP antibody

GFAP (Astrocyte & Neural Stem Cell Marker)

Gene Names
GFAP; ALXDRD
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Synonyms
GFAP, Antibody; GFAP (Astrocyte & Neural Stem Cell Marker); Astrocyte or Intermediate Filament Protein; Glial Fibrillary Acidic Protein (GFAP); anti-GFAP antibody
Ordering
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1/Kappa
Clone Number
GFAP/2076
Specificity
This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
431
Applicable Applications for anti-GFAP antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Recombinant human GFAP protein fragment (around aa 101-200)
Cellular Localization
Cytoplasm
Positive Control
T98G cells (FACS). Human brain lysate (WB). Brain or Astrocytoma (IHC)
Research Areas
Cardiovascular, Endothelial Cell Marker, Neural Stem Cells, Neuroinflammation, Neuroscience, Signal Transduction
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months.
Non-hazardous. No MSDS required

WB (Western Blot)

(Western Blot Analysis of Human Brain, Mouse Brain, Rat Brain, Guinea Pig Brain and Hamster Brain tissue lysates using GFAP Mouse Monoclonal Antibody (GFAP/2076).)

product-image-AAA214651_WB7.jpg WB (Western Blot) (Western Blot Analysis of Human Brain, Mouse Brain, Rat Brain, Guinea Pig Brain and Hamster Brain tissue lysates using GFAP Mouse Monoclonal Antibody (GFAP/2076).)

WB (Western Blot)

(Western Blot Analysis of human brain tissue lysate using GFAP Mouse Monoclonal Antibody (GFAP/2076))

product-image-AAA214651_WB8.jpg WB (Western Blot) (Western Blot Analysis of human brain tissue lysate using GFAP Mouse Monoclonal Antibody (GFAP/2076))

Application Data

(Flow Cytometric Analysis of T98G cells using GFAP Mouse Monoclonal Antibody (GFAP/2076) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)

product-image-AAA214651_AD10.jpg Application Data (Flow Cytometric Analysis of T98G cells using GFAP Mouse Monoclonal Antibody (GFAP/2076) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)

Application Data

(Analysis of Protein Array containing more than 19, 000 full-length human proteins using GFAP Mouse Monoclonal Antibody (GFAP/2076) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

product-image-AAA214651_AD11.jpg Application Data (Analysis of Protein Array containing more than 19, 000 full-length human proteins using GFAP Mouse Monoclonal Antibody (GFAP/2076) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

SDS-PAGE

(SDS-PAGE Analysis Purified GFAP Mouse Monoclonal Antibody (GFAP/2076).Confirmation of Integrity and Purity of Antibody.)

product-image-AAA214651_SDS_PAGE13.jpg SDS-PAGE (SDS-PAGE Analysis Purified GFAP Mouse Monoclonal Antibody (GFAP/2076).Confirmation of Integrity and Purity of Antibody.)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Cerebellum stained with GFAP Mouse Monoclonal Antibody (GFAP/2076).)

product-image-AAA214651_IHC15.jpg IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Cerebellum stained with GFAP Mouse Monoclonal Antibody (GFAP/2076).)
References
McLendon, R.E. and Bigner, D.D. 1994. Immunohistochemistry of the glial fibrillary acidic protein: basic and applied considerations. Brain Pathol. 4: 221-228. Eng, L.F. and Ghirnikar, R.S. 1994. GFAP and astrogliosis. Brain Pathol. 4: 229-237.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~50kDa
NCBI Official Full Name
glial fibrillary acidic protein isoform 2
NCBI Official Synonym Full Names
glial fibrillary acidic protein
NCBI Official Symbol
GFAP
NCBI Official Synonym Symbols
ALXDRD
NCBI Protein Information
glial fibrillary acidic protein
UniProt Protein Name
Glial fibrillary acidic protein
UniProt Gene Name
GFAP
UniProt Synonym Gene Names
GFAP

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Product Notes

The GFAP gfap (Catalog #AAA214651) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GFAP (Astrocyte & Neural Stem Cell Marker) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GFAP can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the GFAP gfap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GFAP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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