ICC (Immunocytochemistry)
(The CCF STTG1 cell line was fixed with 5% acetic acid methanol solution for 30 minutes followed by a 5% BSA/PBS blocking. The cells were permeabilized by 0.1 % Triton x/PBS for 15 minutes and stained with Mouse Anti-Human GFAP-CY3. The slides were mounted with DAPI-Fluormount-G(R))
Mouse anti-Human GFAP Monoclonal Antibody | anti-GFAP antibody
Mouse Anti-Human GFAP-BIOT
Gene Names
GFAP; FLJ45472; GFAP
Reactivity
Human
Applications
ELISA
Synonyms
GFAP, Antibody; Mouse Anti-Human GFAP-BIOT; Mouse Anti-Human GFAP - Biotin; Mouse Anti-Human GFAP; anti-GFAP antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse (BALB/c) IgG2b, kappa
Clone Number
SB61b1
Specificity
Reacts with human GFAP (~ 50 kDa)1
Glial Fibrillary Acid Protein (GFAP) was discovered by Bignami et al 2 as a major fibrous protein of multiple sclerosis plaques. It was subsequently found to be a member of the 10 nm or intermediate filament protein family, specifically the intermediate filament protein family Class III, which also includes peripherin, desmin and vimentin. GFAP is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves.3 Many types of brain tumors, presumably of astrocytic origin, heavily express GFAP. In addition, neural stem cells frequently strongly express GFAP. It is also found in the lens epithelium, Kupffer cells of the liver, in some cells of salivary tumors, and has been reported in erythrocytes. Therefore, antibodies to GFAP are very useful as markers of astrocytic cells and neural stem cells, and for distinguishing neoplasms of astrocytic origin from other neoplasms in the central nervous system.
Although its function is not fully understood, GFAP protein is probably involved in controlling the shape and movement of astrocytes. The protein may also play a significant role in the interactions of astrocytes with other cells, which are required for the formation and maintenance of the myelin layer that covers nerve cells. Additionally, GFAP may assist in maintaining the protective blood-brain barrier.
In adults, GFAP levels increase as a result of the proliferation of astrocytes that occurs in response to a variety of physical, chemical and etiological insults, including Alzheimer's disease, epilepsy and multiple sclerosis. Alexander's disease was recently shown to be caused by point mutations in the protein-coding region of the GFAP gene.4 All forms of Alexander's disease are characterized by the presence of Rosenthal fibers, which are GFAP-containing cytoplasmic inclusions found in astrocytes.
Glial Fibrillary Acid Protein (GFAP) was discovered by Bignami et al 2 as a major fibrous protein of multiple sclerosis plaques. It was subsequently found to be a member of the 10 nm or intermediate filament protein family, specifically the intermediate filament protein family Class III, which also includes peripherin, desmin and vimentin. GFAP is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves.3 Many types of brain tumors, presumably of astrocytic origin, heavily express GFAP. In addition, neural stem cells frequently strongly express GFAP. It is also found in the lens epithelium, Kupffer cells of the liver, in some cells of salivary tumors, and has been reported in erythrocytes. Therefore, antibodies to GFAP are very useful as markers of astrocytic cells and neural stem cells, and for distinguishing neoplasms of astrocytic origin from other neoplasms in the central nervous system.
Although its function is not fully understood, GFAP protein is probably involved in controlling the shape and movement of astrocytes. The protein may also play a significant role in the interactions of astrocytes with other cells, which are required for the formation and maintenance of the myelin layer that covers nerve cells. Additionally, GFAP may assist in maintaining the protective blood-brain barrier.
In adults, GFAP levels increase as a result of the proliferation of astrocytes that occurs in response to a variety of physical, chemical and etiological insults, including Alzheimer's disease, epilepsy and multiple sclerosis. Alexander's disease was recently shown to be caused by point mutations in the protein-coding region of the GFAP gene.4 All forms of Alexander's disease are characterized by the presence of Rosenthal fibers, which are GFAP-containing cytoplasmic inclusions found in astrocytes.
Form/Format
Biotin (BIOT) Conjugate
Sequence Length
432
Applicable Applications for anti-GFAP antibody
ELISA
Immunogen
Recombinant human Glial Fibrillary Acid Protein (GFAP)
Characterization
To ensure acceptable performance, each batch of product is tested by immunohistochemistry and Western blotting to conform to characteristics of a standard reference reagent.
WARNING
Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running waterbefore discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0; store at 2-8 degree C.
HRP conjugate is supplied in 1.0 mL of stock solution in 50% glycerol/50% phosphate buffered saline, pH.7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
Reagents are stable for the period shown on the label if stored as directed.
HRP conjugate is supplied in 1.0 mL of stock solution in 50% glycerol/50% phosphate buffered saline, pH.7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
Reagents are stable for the period shown on the label if stored as directed.
ICC (Immunocytochemistry)
(The CCF STTG1 cell line was fixed with 5% acetic acid methanol solution for 30 minutes followed by a 5% BSA/PBS blocking. The cells were permeabilized by 0.1 % Triton x/PBS for 15 minutes and stained with Mouse Anti-Human GFAP-CY3. The slides were mounted with DAPI-Fluormount-G(R))
ICC (Immunocytochemistry)
(The CCF STTG1 cell line was fixed with 5% acetic acid methanol solution for 30 minutes followed by a 5% BSA/PBS blocking. The cells were permeabilized by 0.1 % Triton x/PBS for 15 minutes and stained with Mouse Anti-Human GFAP-CY3. The slides were mounted with DAPI-Fluormount-G(R))
References
1) Lukas, Z. et al. 1989. Expression of vimentin and glial fibrillary acid protein in human developing spinal cord. Histochem. J. 21:693.
3) Brenner, M. et al. 2001. Mutations in GFAP, encoding glial fibrillary acid protein, are associated with Alexander's Disease. Nat. Genet. 7:118-120.
3) Brenner, M. et al. 2001. Mutations in GFAP, encoding glial fibrillary acid protein, are associated with Alexander's Disease. Nat. Genet. 7:118-120.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
49,880 Da
NCBI Official Full Name
glial fibrillary acidic protein
NCBI Official Synonym Full Names
glial fibrillary acidic protein
NCBI Official Symbol
GFAP
NCBI Official Synonym Symbols
FLJ45472; GFAP
NCBI Protein Information
glial fibrillary acidic protein
UniProt Protein Name
Glial fibrillary acidic protein
UniProt Gene Name
GFAP
UniProt Entry Name
GFAP_HUMAN
Similar Products
Product Notes
The GFAP gfap (Catalog #AAA78621) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human GFAP-BIOT reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GFAP can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the GFAP gfap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GFAP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.