ICC (Immunocytochemistry)
(Immunocytochemical labeling of Girdin in aldehyde fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-Girdin (GM0121). The antibody was detected using goat anti-mouse DyLight 594.)
Mouse anti-Human Girdin Monoclonal Antibody | anti-CCDC88A antibody
Girdin (C-terminus) Antibody
Gene Names
CCDC88A; APE; GIV; GRDN; HkRP1; GIRDIN; KIAA1212
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Protein G chromatography
Synonyms
Girdin, Antibody; Girdin (C-terminus) Antibody; Girdin (C-terminus); Girdin M012; anti-CCDC88A antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
M012
Specificity
Clone M012 mouse monoclonal antibody detects a 250kDa* protein on SDS-PAGE immunoblots of human A431 cells and human brain tissue. The antibody also works for immmunoprecipitation, immunohistochemistry, and immunocytochemistry.
Purity/Purification
Protein G chromatography
Form/Format
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-CCDC88A antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
Clone (M012) was generated from a recombinant protein that included amino acid residues within the C-terminal region of human Girdin.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
ICC (Immunocytochemistry)
(Immunocytochemical labeling of Girdin in aldehyde fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-Girdin (GM0121). The antibody was detected using goat anti-mouse DyLight 594.)
ICC (Immunocytochemistry)
(Immunocytochemical labeling of Girdin in aldehyde fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-Girdin (GM0121). The antibody was detected using goat anti-mouse DyLight 594.)
WB (Western Blot)
(Western blot image of human A431 cell lysates treated with pervanadate (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate Girdin phosphosites (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Girdin (lanes 1 & 2) or rabbit polyclonal anti-Girdin (Tyr-1764), phospho-specific (lanes 3 & 4).)
WB (Western Blot)
(Western blot image of human A431 cell lysates treated with pervanadate (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate Girdin phosphosites (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Girdin (lanes 1 & 2) or rabbit polyclonal anti-Girdin (Tyr-1764), phospho-specific (lanes 3 & 4).)
Related Product Information for anti-CCDC88A antibody
Girdin, a member of the CCDC88 (Hook related protein) family, is an actin binding protein involved with cell migration and maintaining cytoskeletal organization. Girdin has conserved domains at the N- and C-terminus that bind microtubules and actin, respectively. It enhances PI3-kinase dependent phosphorylation of proteins, most notably Akt. This same activity can contribute to tumor proliferation, invasion, and metastasis in breast, ovarian, prostate, and pancreatic tissues. Girdin is phosphorylated at three separate locations: Ser-1416, Ser-1674, and Tyr-1764. Ser-1416 is the primary Akt phosphorylation site, while Cyclin-dependent kinases interact with Girdin and phosphorylate Ser-1674. Multiple receptor tyrosine kinases can bind girdin and phosphorylate Tyr-1764.
References
Hayano S. et al, (2015) J Mol Cell Cardiol. 88:55
Lin, C. (2011) Sci Signal. 4(192): ra64
Jiang, P. (2008) Cancer Res. 1;68(5):1310
Simpson, F. (2005) Traffic. 6(6):442
Lin, C. (2011) Sci Signal. 4(192): ra64
Jiang, P. (2008) Cancer Res. 1;68(5):1310
Simpson, F. (2005) Traffic. 6(6):442
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
216,042 Da
NCBI Official Full Name
girdin isoform 1
NCBI Official Synonym Full Names
coiled-coil domain containing 88A
NCBI Official Symbol
CCDC88A
NCBI Official Synonym Symbols
APE; GIV; GRDN; HkRP1; GIRDIN; KIAA1212
NCBI Protein Information
girdin; Hook-related protein 1; girders of actin filament; girders of actin filaments; AKT-phosphorylation enhancer; g alpha-interacting vesicle-associated protein
UniProt Protein Name
Girdin
UniProt Gene Name
CCDC88A
UniProt Synonym Gene Names
APE; GRDN; KIAA1212; APE; GIV; HkRP1
UniProt Entry Name
GRDN_HUMAN
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Product Notes
The CCDC88A ccdc88a (Catalog #AAA71642) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Girdin (C-terminus) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Girdin can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CCDC88A ccdc88a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Girdin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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