Rat anti-Mouse Gr-1 Monoclonal Antibody | anti-Gr-1 antibody

Rat anti Mouse Gr-1:Amethyst Orange

Cat. Num. AAA12290

• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay
• Purity: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Synonyms: Gr-1, Antibody; Rat anti Mouse Gr-1:Amethyst Orange; Gr-1 antibody | RB6-8C5; anti-Gr-1 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: RB6-8C5
• Purity/Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Form/Format: Phosphate buffered saline; 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin; Purified IgG conjugated to Amethyst Orange - liquid
• Concentration: IgG concentration 0.1mg/ml (varies by lot)

• Applicable Applications for anti-Gr-1 antibody: Flow Cytometry (FC/FACS)
• Application Notes: FC/FACS: Neat to 1/10
• Immunogen: Normal murine bone marrow cells.
• Conjugation: Amethyst Orange
• Preparation and Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C.; ; Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Application Data

(Published customer image:RPE conjugated Rat anti Mouse GR-1 antibody, clone RB6-8C5 used for the evaluation of GR-1 (Ly6G) expression on murine uterine derived neutrophils by flow cytometry.Image caption:Immunophenotyping of uterin)

Application Data

(Published customer image:Rat anti Mouse Gr-1 antibody, clone RB6-8C5 used for the evaluation of Gr-1 expression on circulating monocytes from mouse blood by flow cytometry.Image caption:Effect of CD206+ M2 macrophage depletion on collagen depo)

Application Data

(Published customer image:Rat anti Mouse Gr-1 antibody, clone RB6-8C5 used for the identification of infiltrating neutrophils in murine lung tissue sections by immunofluorescenceImage caption:Representative images of lung tissue from cont)

Application Data

(Published customer image:Rat anti Mouse Gr-1 antibody used for the visualisation of infiltrating granulocytes in a murine model of ureteral obstruction induced kidney fibrosis by immunohistochemistry on formalin fixed, paraffin embedded tiss)

Application Data

(Published customer image:Rat anti Mouse Gr-1 antibody, clone RB6-8C5 used for the identification of neutrophils by immunofluorescence.Image caption:S. typhimurium-Infected Macrophages Containing Phagocytosed Neutrophils and T Cells Confocal fl)

Application Data

(Published customer image:Rat anti Mouse Gr-1 antibody, clone RB6-8C5 used for the evaluation of Gr-1 expression on circulating monocytes from mouse blood by flow cytometry.Image caption:Depletion of the circulating monocyte and Gr1lo and F4/80)

Application Data

(Figure A. RPE conjugated Rat anti Mouse CD11b and Alexa Fluor 647 conjugated Rat IgG2b isotype control. Figure B. RPE conjugated Rat anti Mouse CD11b and Alexa Fluor 647 conjugated Rat anti Mouse Gr-1 . All experiments p)

Application Data

(Figure A. Pacific Blue conjugated Rat anti Mouse CD11b and FITC conjugated Rat IgG2b isotype control . Figure B. Pacific Blue conjugated Rat anti Mouse CD11b and FITC conjugated Rat anti Mouse GR-1 . All experimen)

Application Data

(Figure A. Pacific Blue conjugated Rat anti Mouse CD11b and Alexa Fluor700 conjugated Rat IgG2b isotype control . Figure B. Pacific Blue conjugated Rat anti Mouse CD11b and Alexa Fluor700 conjugated Rat anti Mouse GR-1 (M)

Application Data

(Figure A. RPE conjugated Rat anti Mouse CD11b and Pacific Blue conjugated Rat IgG2b isotype control . Figure B. RPE conjugated Rat anti Mouse CD11b and Pacific Blue conjugated Rat anti Mouse GR-1 . All experimen)

Related Product Information for anti-Gr-1 antibody

Rat anti Mouse Gr-1 antibody, clone RB6-8C5 recognizes the mouse Gr-1 antigen, a ~21-25kDa GPI anchored cell surface protein bearing a single uPAR/Ly6 domain that belongs to the Ly-6 family of proteins (Lee et al. 2013). Rat anti Mouse Gr-1 antibody, clone RB6-8C5 reacts predominantly with the Ly-6G protein but weaker reactivity with the Ly-6C protein has been reported (Fleming et al. 1993). However, other observations dispute the cross-reactivity of clone RB6-8C5 with the Ly-6C protein with the alternative explanation that certain sub-populations of bone marrow cells simultaneously express both Ly-6C and Ly-6G (Nagendra et al. 2007)

The Gr-1 antigen is primarily a marker of myeloid differentiation. In the bone marrow the level of Gr-1 expression is low on immature myeloblasts and increases as the myeloid cells mature to granulocytes. Gr-1 is also expressed on macrophages and transiently on differentiating monocytes.

Rat anti Mouse Gr-1 antibody, clone RB6-8C5 has been used successfully for the depletion of mature neutrophils in vivo (Czuprynski et al 1994, Daley et al. 2008).

NCBI and Uniprot Product Information

• NCBI GI #: 547876
• UniProt Accession #: P35461
• Molecular Weight: 11,798 Da
• NCBI Official Full Name: Lymphocyte antigen 6G
• UniProt Protein Name: Lymphocyte antigen 6G
• UniProt Gene Name: Ly6g
• UniProt Synonym Gene Names: Ly-6G
• UniProt Entry Name: LY6G_MOUSE