Rat GRP94 / HSP90B1 Monoclonal Antibody | anti-HSP90B1 antibody

GRP94 / HSP90B1 (Endoplasmic Reticulum Marker) Rat Monoclonal Antibody

Cat. Num. AAA62837

• Gene Names: HSP90B1; ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m
• Reactivity: Human, Monkey, Horse, Cow, Sheep, Pig, Rabbit, Dog, Rat, Mouse, Hamster, Guinea Pig, Chicken, Xenopus laevis
• Applications: Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: GRP94 / HSP90B1, Antibody; GRP94 / HSP90B1 (Endoplasmic Reticulum Marker) Rat Monoclonal Antibody; Glucose Regulated Protein 94; 94kDa glucose-regulated protein, Endoplasmin, Endothelial cell (HBMEC) glycoprotein (ECGP), Heat shock protein 90kDa beta member 1 (HSP90B1), Stress inducible tumor rejection antigen GP96, TRA1, Tumor rejection antigen-1 (gp96); anti-HSP90B1 antibody

• Host: Rat
• Reactivity: Human, Monkey, Horse, Cow, Sheep, Pig, Rabbit, Dog, Rat, Mouse, Hamster, Guinea Pig, Chicken, Xenopus laevis
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: 9G10.F8.2
• Specificity: Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grp s are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
• Sequence Length: 803

• Applicable Applications for anti-HSP90B1 antibody: IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Cellular Localization: Cytoplasmic and nuclear
• Immunogen: Purified glucose regulated protein 94 (grp94) from chicken oviducts
• Hu-Chromosome Location: 12q23.3
• Positive Control: U20S cells. About 50% of breast tumors express grp94 protein
• Preparation and Storage: Antibody with azide - store at 2 to 8°C. Non-hazardous. No MSDS required.

FCM/FACS (Flow Cytometry)

(Flow Cytometric Analysis of PFA-fixed HePG2 cells using GRP94 Rat Monoclonal Antibody (9G10.F8.2) followed by Goat anti-Rat- IgG-CF488 (Blue); Isotype Control (Red).)

IF (Immunofluorescence)

(Immunofluorescence Analysis of HePG2 cells labeling GRP94 with GRP94 Rat Monoclonal Antibody (9G10.F8.2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot.(Red).)

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GRP94 Rat Monoclonal Antibody (9G10.F8.2).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GRP94 Monoclonal Antibody (9G10.F8.2).)

References

Sorger, P.K. et al. J. Mol. Biol. 194: 341-344 (1987). Tandon, A.K. et.al. Breast Cancer Res. and Treat. 16: 146 (1990).

NCBI and Uniprot Product Information

• NCBI GI #: 4507677
• NCBI GeneID: 7184
• NCBI Accession #: NP_003290.1
• NCBI GenBank Nucleotide #: NM_003299.2
• UniProt Accession #: P14625; Q96A97
• Molecular Weight: 94kDa
• NCBI Official Full Name: endoplasmin
• NCBI Official Synonym Full Names: heat shock protein 90 beta family member 1
• NCBI Official Symbol: HSP90B1
• NCBI Official Synonym Symbols: ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m
• NCBI Protein Information: endoplasmin
• UniProt Protein Name: Endoplasmin
• UniProt Gene Name: HSP90B1
• UniProt Synonym Gene Names: GRP94; TRA1; GRP-94
• UniProt Entry Name: ENPL_HUMAN