FCM/FACS (Flow Cytometry)
(Flow cytometry analysis of GSTT1 in Hep3B cell line, staining at 2-5ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.)
Mouse anti-Human GSTT1 Monoclonal Antibody | anti-GSTT1 antibody
GSTT1 antibody
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
GSTT1, Antibody; GSTT1 antibody; Glutathione S-transferase theta 1; GSTT1; GST class-theta-1; Glutathione transferase T1-1; Glutathione S-transferase theta 1 EC 2.5.1.18; Glutathione S transferase 5; Glutathione S transferase theta 1; GST class theta 1; Glutathione transferase T1 1; GST 5 5; GST CL1,; anti-GSTT1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT38D11
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
240
Applicable Applications for anti-GSTT1 antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human GSTT1 (1-240aa) purified from E. coli
Usage
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Preparation and Storage
Can be stored at +2°C to +8°C for 1 week.
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometry analysis of GSTT1 in Hep3B cell line, staining at 2-5ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.)
FCM/FACS (Flow Cytometry)
(Flow cytometry analysis of GSTT1 in Hep3B cell line, staining at 2-5ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.)
IF (Immunofluorescence)
(ICC/IF analysis of GSTT1 in HepG2 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GSTT1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IF (Immunofluorescence)
(ICC/IF analysis of GSTT1 in HepG2 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GSTT1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot)
(The recombinant human GSTT1 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GSTT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: GSTT1 Recombinant protein Lane 2.: HeLa cell lysate Lane 3.: 293T cell lysate Lane 4.: Jurkat cell lysate Lane 5.: HL-60 cell lysate)
WB (Western Blot)
(The recombinant human GSTT1 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GSTT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: GSTT1 Recombinant protein Lane 2.: HeLa cell lysate Lane 3.: 293T cell lysate Lane 4.: Jurkat cell lysate Lane 5.: HL-60 cell lysate)
Related Product Information for anti-GSTT1 antibody
Glutathione S-transferase theta 1 (GSTT1) is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes (alpha, mu, pi, theta, and zeta). The theta class includes GSTT1 and GSTT2. The GSTT1 and GSTT2 share 55% amino acid sequence identity and both of them were claimed to have an important role in human carcinogenesis. The GSTT1 gene is located approximately 50kb away from the GSTT2 gene. The GSTT1 and GSTT2 genes have a similar structure, being composed of five exons with identical exon/intron boundaries.
Product Categories/Family for anti-GSTT1 antibody
References
Webb G., et al. (1996) Genomics. 33 (1): 121-123.; Mainwaring GW., et al. (1996) Biochem J. 318: 297-303.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Glutathione S-transferase theta 1
NCBI Official Synonym Full Names
glutathione S-transferase theta 1
NCBI Official Symbol
GSTT1
NCBI Protein Information
glutathione S-transferase theta-1
UniProt Protein Name
Glutathione S-transferase theta-1
UniProt Gene Name
GSTT1
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Product Notes
The GSTT1 gstt1 (Catalog #AAA48193) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GSTT1 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GSTT1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the GSTT1 gstt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GSTT1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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