FCM/FACS (Flow Cytometry)
(GSTT1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human GSTT1 Polyclonal Antibody | anti-GSTT1 antibody
GSTT1 Antibody (N-term)
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
GSTT1, Antibody; GSTT1 Antibody (N-term); Glutathione S-transferase theta-1; GST class-theta-1; Glutathione transferase T1-1; GSTT1; anti-GSTT1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This GSTT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-34 amino acids from the N-terminal region of human GSTT1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
7-34
Sequence Length
122
Applicable Applications for anti-GSTT1 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(GSTT1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(GSTT1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot analysis of GSTT1 (arrow) using rabbit polyclonal GSTT1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GSTT1 gene.)
WB (Western Blot)
(Western blot analysis of GSTT1 (arrow) using rabbit polyclonal GSTT1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GSTT1 gene.)
WB (Western Blot)
(Anti-GSTT1 Antibody (N-term)at 1:2000 dilution + human lung lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 27 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-GSTT1 Antibody (N-term)at 1:2000 dilution + human lung lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 27 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-GSTT1 antibody
Glutathione S-transferase (GST) theta 1 (GSTT1) is a
member of a superfamily of proteins that catalyze the conjugation
of reduced glutathione to a variety of electrophilic and
hydrophobic compounds. Human GSTs can be divided into five main
classes: alpha, mu, pi, theta, and zeta. The theta class includes
GSTT1 and GSTT2. The GSTT1 and GSTT2 share 55% amino acid sequence
identity and both of them were claimed to have an important role in
human carcinogenesis. The GSTT1 gene is located approximately 50kb
away from the GSTT2 gene. The GSTT1 and GSTT2 genes have a similar
structure, being composed of five exons with identical exon/intron
boundaries.
member of a superfamily of proteins that catalyze the conjugation
of reduced glutathione to a variety of electrophilic and
hydrophobic compounds. Human GSTs can be divided into five main
classes: alpha, mu, pi, theta, and zeta. The theta class includes
GSTT1 and GSTT2. The GSTT1 and GSTT2 share 55% amino acid sequence
identity and both of them were claimed to have an important role in
human carcinogenesis. The GSTT1 gene is located approximately 50kb
away from the GSTT2 gene. The GSTT1 and GSTT2 genes have a similar
structure, being composed of five exons with identical exon/intron
boundaries.
Product Categories/Family for anti-GSTT1 antibody
References
Palli, D., et al. Mutagenesis 25(6):569-575(2010)
Henderson, A.J., et al. Thorax 65(10):897-902(2010)
Filonzi, L., et al. Birth Defects Res. Part A Clin. Mol. Teratol. 88(9):743-747(2010)
Smith, G., et al. Pharmacogenet. Genomics (2010) In press :
Bid, H.K., et al. J Postgrad Med 56(3):176-181(2010)
Henderson, A.J., et al. Thorax 65(10):897-902(2010)
Filonzi, L., et al. Birth Defects Res. Part A Clin. Mol. Teratol. 88(9):743-747(2010)
Smith, G., et al. Pharmacogenet. Genomics (2010) In press :
Bid, H.K., et al. J Postgrad Med 56(3):176-181(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27335
NCBI Official Full Name
glutathione S-transferase theta-1 isoform a
NCBI Official Synonym Full Names
glutathione S-transferase theta 1
NCBI Official Symbol
GSTT1
NCBI Protein Information
glutathione S-transferase theta-1
UniProt Protein Name
Glutathione S-transferase theta-1
UniProt Gene Name
GSTT1
UniProt Entry Name
GSTT1_HUMAN
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Product Notes
The GSTT1 gstt1 (Catalog #AAA285038) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 7-34. The GSTT1 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GSTT1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the GSTT1 gstt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GSTT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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