FCM/FACS (Flow Cytometry)
(Flow Cytometric Analysis of PFA-fixed HeLa cells. GTF2H2 Mouse Monoclonal Antibody (PCRP-GTF2H2-1B9) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).)
Mouse anti-Human GTF2H2/BTF2/TFIIH Monoclonal Antibody | anti-GTF2H2 antibody
GTF2H2/BTF2/TFIIH Basal Transcription Factor Mouse Monoclonal Antibody [Clone PCRP-GTF2H2-1B9]
Gene Names
GTF2H2; p44; BTF2; TFIIH; BTF2P44; T-BTF2P44
Reactivity
Human
Applications
Immunofluorescence, Flow Cytometry, Functional Assay
Synonyms
GTF2H2/BTF2/TFIIH, Antibody; GTF2H2/BTF2/TFIIH Basal Transcription Factor Mouse Monoclonal Antibody [Clone PCRP-GTF2H2-1B9]; GTF2H2/BTF2/TFIIH Basal Transcription Factor; Basic transcription factor 2 44kDa subunit; BTF2; BTF2-p44; BTF2P44; General transcription factor IIH, polypeptide 2, 44kDa; T-BTF2P44; TFIIH basal transcription factor complex p44 subunit; anti-GTF2H2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse/IgG1
Clone Number
PCRP-GTF2H2-1B9
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
Purified Ab with BSA and Azide at 200ug/ml
OR
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)
OR
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)
Applicable Applications for anti-GTF2H2 antibody
IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Immunogen
Recombinant full-length human GTF2H2 protein
Cellular Localization
Nucleus
Positive Control
HeLa or MCF7 cells.
Hu-chromosome Location
5q13.2
Unigene
607501
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C
Antibody without azide: store at -20 to -80 degree C
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
FCM/FACS (Flow Cytometry)
(Flow Cytometric Analysis of PFA-fixed HeLa cells. GTF2H2 Mouse Monoclonal Antibody (PCRP-GTF2H2-1B9) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).)
FCM/FACS (Flow Cytometry)
(Flow Cytometric Analysis of PFA-fixed HeLa cells. GTF2H2 Mouse Monoclonal Antibody (PCRP-GTF2H2-1B9) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).)
Related Product Information for anti-GTF2H2 antibody
Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in the formation of a multiprotein complex containing several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFII. TFIIH (or BTF2) is a multisubunit transcription/DNA repair factor that possesses several enzymatic activities. The core of TFIIH is composed of five subunits, designated p89 (XPB or ERCC3), p62, p52, p44 and p34. Additional subunits of the TFIIH complex are p80 (XPD or ERCC2) and the ternary kinase complex composed of Cdk7, cyclin H and Mat1. Both p89 and p80 have ATP-dependent helicase activity. The p62, p52 and p44 subunits have been shown to be involved in nucleotide excision repair.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44,419 Da
NCBI Official Full Name
general transcription factor IIH subunit 2
NCBI Official Synonym Full Names
general transcription factor IIH, polypeptide 2, 44kDa
NCBI Official Symbol
GTF2H2
NCBI Official Synonym Symbols
p44; BTF2; TFIIH; BTF2P44; T-BTF2P44
NCBI Protein Information
general transcription factor IIH subunit 2; BTF2 p44; basic transcription factor 2 44 kDa subunit; general transcription factor IIH polypeptide 2; TFIIH basal transcription factor complex p44 subunit; general transcription factor IIH, polypeptide 2, 44kD
UniProt Protein Name
General transcription factor IIH subunit 2
UniProt Gene Name
GTF2H2
UniProt Synonym Gene Names
BTF2P44; BTF2 p44
UniProt Entry Name
TF2H2_HUMAN
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Product Notes
The GTF2H2 gtf2h2 (Catalog #AAA215693) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GTF2H2/BTF2/TFIIH Basal Transcription Factor Mouse Monoclonal Antibody [Clone PCRP-GTF2H2-1B9] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GTF2H2/BTF2/TFIIH can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the GTF2H2 gtf2h2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GTF2H2/BTF2/TFIIH, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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