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ICC (Immunocytochemistry)
(Localization of human GTF2IRD1 in HeLa cells.HeLa cells transiently transfected with the myc-tagged GTF2IRD1 expression vector were fixed and stained with anti- GTF2IRD1 (BMR00190) and Hoechst 33342. Human GTF2IRD1 fluorescence signals were observed exc)
Mouse anti-Human GTF2IRD1 Monoclonal Antibody | anti-GTF2IRD1 antibody
Mouse monoclonal antibody Anti-Human GTF2IRD1
Gene Names
GTF2IRD1; BEN; WBS; GTF3; RBAP2; CREAM1; MUSTRD1; WBSCR11; WBSCR12; hMusTRD1alpha1
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunocytochemistry, ELISA
Synonyms
GTF2IRD1, Antibody; Mouse monoclonal antibody Anti-Human GTF2IRD1; GTF2I repeat domain containing 1 (GTF2IRD1); GTF3; RBAP2; CREAM1; MUSTRD1; WBSCR11; WBSCR12; hMusTRD1alpha1; anti-GTF2IRD1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
GTF5I102
Concentration
100 ug/ml (1.0 ml) (varies by lot)
Sequence Length
3471
Applicable Applications for anti-GTF2IRD1 antibody
WB (Western Blot), IP (Immunoprecipitation), ICC (Immunocytochemistry), ELISA
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.
ICC (Immunocytochemistry)
(Localization of human GTF2IRD1 in HeLa cells.HeLa cells transiently transfected with the myc-tagged GTF2IRD1 expression vector were fixed and stained with anti- GTF2IRD1 (BMR00190) and Hoechst 33342. Human GTF2IRD1 fluorescence signals were observed exc)
ICC (Immunocytochemistry)
(Localization of human GTF2IRD1 in HeLa cells.HeLa cells transiently transfected with the myc-tagged GTF2IRD1 expression vector were fixed and stained with anti- GTF2IRD1 (BMR00190) and Hoechst 33342. Human GTF2IRD1 fluorescence signals were observed exc)
IP (Immunoprecipitation)
(Immunoprecipitation: RIPA lysate of HeLa cells transfected with vector (lanes 1, 3) or myc-tagged GTF2IRD1 (lanes 2, 4) was incubated with anti-GTF2IRD1 (BMR00190) conjugated with protein G sepharose. Immunocomplex was analyzed by Western blot with anti-mPredicted molecular weight: 106 kDa)
IP (Immunoprecipitation)
(Immunoprecipitation: RIPA lysate of HeLa cells transfected with vector (lanes 1, 3) or myc-tagged GTF2IRD1 (lanes 2, 4) was incubated with anti-GTF2IRD1 (BMR00190) conjugated with protein G sepharose. Immunocomplex was analyzed by Western blot with anti-mPredicted molecular weight: 106 kDa)
WB (Western Blot)
(Detection of human GTF2IRD1 by Western blot.Sample: Whole cell lysate was obtained from HeLa cells transiently transfected with vector (lanes 1, 3) or myc-tagged human GTF2IRD1 (lanes 2, 4). Primary antibodies: anti-myc tag (1 ug/ml) (lanes 1, 2) or antPredicted molecular weight: 106 kDa)
WB (Western Blot)
(Detection of human GTF2IRD1 by Western blot.Sample: Whole cell lysate was obtained from HeLa cells transiently transfected with vector (lanes 1, 3) or myc-tagged human GTF2IRD1 (lanes 2, 4). Primary antibodies: anti-myc tag (1 ug/ml) (lanes 1, 2) or antPredicted molecular weight: 106 kDa)
Quality Control #2
(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control #2
(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-GTF2IRD1 monoclonal antibody.)
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-GTF2IRD1 monoclonal antibody.)
Related Product Information for anti-GTF2IRD1 antibody
The protein encoded by this gene contains five GTF2I-like repeats and each repeat possesses a potential helix-loop-helix (HLH) motif. It may have the ability to interact with other HLH-proteins and function as a transcription factor or as a positive transcriptional regulator under the control of Retinoblastoma protein. This gene is deleted in Williams-Beuren syndrome, a multisystem developmental disorder caused by deletion of multiple genes at 7q11.23. Alternative splicing of this gene generates at least 2 transcript variants. [NCBI Entrez Gene Summary]
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
107,979 Da
NCBI Official Full Name
Homo sapiens GTF2I repeat domain containing 1 (GTF2IRD1), transcript variant 1, mRNA
NCBI Official Synonym Full Names
GTF2I repeat domain containing 1
NCBI Official Symbol
GTF2IRD1
NCBI Official Synonym Symbols
BEN; WBS; GTF3; RBAP2; CREAM1; MUSTRD1; WBSCR11; WBSCR12; hMusTRD1alpha1
NCBI Protein Information
general transcription factor II-I repeat domain-containing protein 1; USE B1-binding protein; Williams-Beuren syndrome chromosome region 11; binding factor for early enhancer; general transcription factor 3; general transcription factor III; muscle TFII-I repeat domain-containing protein 1 alpha 1; slow-muscle-fiber enhancer-binding protein; williams-Beuren syndrome chromosomal region 12 protein
UniProt Protein Name
General transcription factor II-I repeat domain-containing protein 1
UniProt Gene Name
GTF2IRD1
UniProt Entry Name
GT2D1_HUMAN
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Product Notes
The GTF2IRD1 gtf2ird1 (Catalog #AAA36322) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse monoclonal antibody Anti-Human GTF2IRD1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GTF2IRD1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), ICC (Immunocytochemistry), ELISA. Researchers should empirically determine the suitability of the GTF2IRD1 gtf2ird1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GTF2IRD1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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